Investigation of DNA Sequences Utilizing Frequency-Selective Nanopore Structures

被引:0
作者
Hilal-Alnaqbi, Ali [4 ]
Al Ahmad, Mahmoud [1 ,5 ]
Rizvi, Tahir A. [2 ,5 ]
Mustafa, Farah [3 ,5 ]
机构
[1] United Arab Emirates Univ, Coll Engn, Dept Elect Engn, Al Ain, U Arab Emirates
[2] United Arab Emirates Univ, Coll Med & Hlth Sci, Dept Microbiol & Immunol, Al Ain, U Arab Emirates
[3] United Arab Emirates Univ, Coll Med & Hlth Sci, Dept Biochem, Al Ain, U Arab Emirates
[4] United Arab Emirates Univ, Coll Engn, Dept Mech Engn, POB 15551, Al Ain, U Arab Emirates
[5] United Arab Emirates Univ, Zayed Bin Sultan Ctr, Hlth Sci Div, Al Ain, U Arab Emirates
来源
BIOINFORMATICS AND BIOMEDICAL ENGINEERING, IWBBIO 2017, PT II | 2017年 / 10209卷
关键词
DNA; Sequencing; Frequency; Nanopore; MICROWAVE;
D O I
10.1007/978-3-319-56154-7_1
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Newer methodologies that are quick, label-free, reliable, and low-cost for DNA sequencing and identification are currently being explored. High frequency based-scattering parameters provide a reliable measurement platform and technique to characterize DNA bases. Using a modeling approach, this work investigates the utilization of high frequency-selective structure coupled with nanopore technology for nucleotide identification and sequencing. The model envisions a coplanar waveguide structure harboring a small hole with an internal diameter of the order of several nanometers to demonstrate the potential use of high frequency to identify and sequence DNA. When DNA molecule enters the pore, it should cause disturbance in the electromagnetic field. This disturbance should result in a shift in the resonance frequency and its corresponding characteristics, thus enabling nucleotide identification. The frequency response of four different single DNA strands composed exclusively of either A, C, G or T were measured and characterized to extract the corresponding dielectric constants and their corresponding base paired strands. These dielectric constant values were then used to model the presence of the corresponding DNA molecules in the nanopore. The conducted simulations revealed distinctions between the single and double-stranded DNA molecules due to their different and distinct electrical properties.
引用
收藏
页码:3 / 11
页数:9
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