An electrochemical method for making enzyme microsensors. Application to the detection of dopamine and glutamate

被引：96

作者：

Cosnier, S ^{[1
]}

Innocent, C ^{[1
]}

Allien, L ^{[1
]}

Poitry, S ^{[1
]}

Tsacopoulos, M ^{[1
]}

机构：

[1] UNIV GENEVA,CTR MED,EXPT OPHTHALMOL LAB,CH-1211 GENEVA 4,SWITZERLAND

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 05期

关键词：

D O I：

10.1021/ac960841h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method of microbiosensor fabrication is described. It is based on the electrochemical polymerization of an enzyme-amphiphilic pyrroleammonium solution on the surface of a microelectrode in the absence of supporting electrolyte. By trapping glutamate oxidase (GMO) or polyphenol oxidase (PPO) in such polypyrrole films, we made microbiosensors for the amperometric determination of glutamate or dopamine, respectively. The response of the GMO microelectrode to glutamate was based on the amperometric detection of the enzymically generated hydrogen peroxide at 0.6 V vs SCE. The detection limit and sensitivity of this microbiosensor were 1 mu M and 32 mA M(-1) cm(-2), respectively. The response of the PPO microelectrode to dopamine was based on the amperometric detection of the enzymically generated quinoid product at -0.2 V. The calibration range for dopamine measurement was 5 x 10(-8)-8 x 10(-5) M and the detection limit and sensitivity were 5 x 10(-8) M and 59 mA M(-1) cm(-2), respectively.

引用

页码：968 / 971

页数：4

共 32 条

[1] CHARACTERIZATION OF GLUCOSE MICROSENSORS FOR INTRACELLULAR MEASUREMENTS [J].

ABE, T ;

LAU, YY ;

EWING, AG .

ANALYTICAL CHEMISTRY, 1992, 64 (18) :2160-2163

[2]

BARTLETT PN, 1993, J ELECTROANAL CHEM, V362, P1

[3] Polyphenol oxidase-catechol: An electroenzymatic model system for characterizing the performance of matrices for biosensors [J].

Besombes, JL ;

Cosnier, S ;

Labbe, P .

TALANTA, 1996, 43 (09) :1615-1619

[4] MICROPOROUS ALUMINUM-OXIDE FILMS AT ELECTRODES .7. MEDIATION OF THE CATALYTIC ACTIVITY OF GLUCOSE-OXIDASE VIA LATERAL DIFFUSION OF A FERROCENE AMPHIPHILE IN A BILAYER ASSEMBLY [J].

BOURDILLON, C ;

MAJDA, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (05) :1795-1799

[5] Microelectrodes for the measurement of catecholamines in biological systems [J].

Cahill, PS ;

Walker, QD ;

Finnegan, JM ;

Mickelson, GE ;

Travis, ER ;

Wightman, RM .

ANALYTICAL CHEMISTRY, 1996, 68 (18) :3180-3186

[6] ELECTROPOLYMERIZATION OF CATIONIC AMPHIPHILIC PYRROLE DERIVATIVES ON ELECTRODES - EVIDENCE FOR ENVIRONMENTAL-EFFECTS ON REDOX POTENTIALS OF TRAPPED ANIONS [J].

COCHEGUERENTE, L ;

DERONZIER, A ;

GALLAND, B ;

MOUTET, JC ;

LABBE, P ;

REVERDY, G ;

CHEVALIER, Y ;

AMHRAR, J .

LANGMUIR, 1994, 10 (02) :602-610

[7] CONTROLLED ELECTROCHEMICAL PREPARATION OF ENZYMATIC LAYERS FOR THE DESIGN OF AMPEROMETRIC BIOSENSORS [J].

COCHEGUERENTE, L ;

COSNIER, S ;

INNOCENT, C ;

MAILLEY, P ;

MOUTET, JC ;

MORELIS, RM ;

LECA, B ;

COULET, PR .

ELECTROANALYSIS, 1993, 5 (08) :647-652

[8] GLUTAMATE OXIDASE ENZYME ELECTRODES - MICROSENSORS FOR NEUROTRANSMITTER DETERMINATION USING ELECTROCHEMICALLY POLYMERIZED PERMSELECTIVE FILMS [J].

COOPER, JM ;

FOREMAN, PL ;

GLIDLE, A ;

LING, TW ;

PRITCHARD, DJ .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 388 (1-2) :143-149

[9] DETECTION OF GALACTOSE AND LACTOSE BY A POLY(AMPHIPHILIC PYRROLE)-GALACTOSE OXIDASE ELECTRODE [J].

COSNIER, S ;

INNOCENT, C .

ANALYTICAL LETTERS, 1994, 27 (08) :1429-1442

[10] A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR [J].

COSNIER, S ;

INNOCENT, C .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1993, 31 (02) :147-160

← 1 2 3 4 →