Selective extraction of cordycepin from Cordyceps militaris - optimisation, kinetics and equilibrium studies

被引：4

作者：

Sornchaithawatwong, Chayanid ^{[1
]}

Kunthakudee, Naphaphan ^{[2
]}

Sunsandee, Niti ^{[3
]}

Ramakul, Prakorn ^{[2
]}

机构：

[1] Srinakharinwirot Univ, Fac Pharm, Dept Pharmaceut Chem, Nakhonnayok, Thailand

[2] Silpakorn Univ, Fac Engn & Ind Technol, Dept Chem Engn, Nakhon Pathom 73000, Thailand

[3] Chulalongkorn Univ, Fac Engn, Dept Chem Engn, Bangkok, Thailand

来源：

INDIAN CHEMICAL ENGINEER | 2022年 / 64卷 / 01期

关键词：

Cordycepin; Cordyceps militaris; extraction; RP-HPLC; POLYSACCHARIDE; IDENTIFICATION; PURIFICATION; ANTITUMOR; SINENSIS;

D O I：

10.1080/00194506.2020.1776163

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Cordycepin was separated successfully from Cordyceps militaris using extraction with a mixture of ethanol and water. Optimum temperature, composition of ethanol in water, solvent/solid ratio and pH of solvent were determined by a central composite design (CCD) with respect to extraction yield. The highest yield for cordycepin (96%) was predicted by the regression model and validated by comparison with the experimental results, showing good agreement. The RP-HPLC method was applied to analyse cordycepin from Cordyceps militaris extracts, and 100% purity of cordycepin was achieved. The extraction characteristics were investigated in terms of equilibrium and kinetics. The second-order kinetics correlates well with the experimental data.

引用

页码：1 / 13

页数：13

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