The self-association of flavin mononucleotide (FMN2-) as determined by H-1 NMR shift measurements

被引：24

作者：

Bastian, M ^{[1
]}

Sigel, H ^{[1
]}

机构：

[1] UNIV BASEL,INST INORGAN CHEM,CH-4056 BASEL,SWITZERLAND

来源：

BIOPHYSICAL CHEMISTRY | 1997年 / 67卷 / 1-3期

关键词：

self-association constants; concentration effects on self-stacking; isoalloxazine residue; isodesmic model for indefinite non-cooperative self-association; self-stacking;

D O I：

10.1016/S0301-4622(97)00012-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentration dependence of the H-1 NMR chemical upfield shifts of the protons H6, H9, H7 alpha, and H8 alpha of the 7,8-dimethylisoalloxazine residue of flavin mononucleotide (FMN2-) has been measured and the self-stacking tendency of FMN2- was quantified with the isodesmic model of indefinite non-cooperative self-association. The stacking tendency of FMN2- is considerable and described in the concentration range of 0.0025-O.1 M with the indicated model by K = 27 +/- 15M(-1) (25 degrees C; I = 0.1-0.3 M). This result is compared with related ones from the literature. The caveats regarding the self-stacking properties of FMN2- and their dependence on the concentration are discussed. (C) 1997 Elsevier Science B.V.

引用

页码：27 / 34

页数：8

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