The COMPASS Subunit Spp1 Links Histone Methylation to Initiation of Meiotic Recombination

被引：152
作者：
Acquaviva, Laurent [1 ]
Szekvoelgyi, Lorant [2 ,4 ]
Dichtl, Bernhard [3 ]
Dichtl, Beatriz Solange [3 ]
Saint Andre, Christophe de La Roche [1 ]
Nicolas, Alain [2 ]
Geli, Vincent [1 ]
机构：
[1] Aix Marseille Univ, Inst Paoli Calmettes, Marseille Canc Res Ctr CRCM, INSERM,CNRS,U1068,UMR7258, F-13009 Marseille, France
[2] Univ Paris 06, CNRS, Inst Curie, Ctr Rech,UMR3244, F-75248 Paris, France
[3] Deakin Univ, Sch Life & Environm Sci, Ctr Cellular & Mol Biol, Melbourne, Vic 3125, Australia
[4] Univ Debrecen, Med & Hlth Sci Ctr, Dept Biophys & Cell Biol, H-4012 Debrecen, Hungary
来源：
SCIENCE | 2013年 / 339卷 / 6116期
基金：
瑞士国家科学基金会;
关键词：
STRAND BREAK FORMATION; H3; TRIMETHYLATION; GENE-EXPRESSION; SITES; PROMOTER; FEATURES; HOTSPOTS; COMPLEX; PRDM9; ONSET;
D O I：
10.1126/science.1225739
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions.
引用
收藏
页码：215 / 218
页数：4
相关论文
共 24 条
  • [1] PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice
    Baudat, F.
    Buard, J.
    Grey, C.
    Fledel-Alon, A.
    Ober, C.
    Przeworski, M.
    Coop, G.
    de Massy, B.
    [J]. SCIENCE, 2010, 327 (5967) : 836 - 840
  • [2] Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation
    Blat, Y
    Protacio, RU
    Hunter, N
    Kleckner, N
    [J]. CELL, 2002, 111 (06) : 791 - 802
  • [3] Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites
    Borde, Valerie
    Robine, Nicolas
    Lin, Waka
    Bonfils, Sandrine
    Geli, Vincent
    Nicolas, Alain
    [J]. EMBO JOURNAL, 2009, 28 (02) : 99 - 111
  • [4] Protein interactions within the Set1 complex and their roles in the regulation of histone 3 lysine 4 methylation
    Dehe, Pierre-Marie
    Dichtl, Bernhard
    Schaft, Daniel
    Roguev, Assen
    Pamblanco, Merce
    Lebrun, Regine
    Rodriguez-Gil, Alfonso
    Mkandawire, Msau
    Landsberg, Katarina
    Shevchenko, Anna
    Shevchenko, Andrej
    Rosaleny, Lorena E.
    Tordera, Vicente
    Chavez, Sebastian
    Stewart, A. Francis
    Geli, Vincent
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (46) : 35404 - 35412
  • [5] Cyclin-dependent kinase directly regulates initiation of meiotic recombination
    Henderson, Kiersten A.
    Kee, Kehkooi
    Maleki, Shohreh
    Santini, Paul A.
    Keeney, Scott
    [J]. CELL, 2006, 125 (07) : 1321 - 1332
  • [6] Keeney Scott, 2008, V2, P81, DOI 10.1007/7050_2007_026
  • [7] Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6
    Lardenois, Aurelie
    Liu, Yuchen
    Walther, Thomas
    Chalmel, Frederic
    Evrard, Bertrand
    Granovskaia, Marina
    Chu, Angela
    Davis, Ronald W.
    Steinmetz, Lars M.
    Primig, Michael
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (03) : 1058 - 1063
  • [8] Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation
    Li, Jing
    Hooker, Gillian W.
    Roeder, G. Shirleen
    [J]. GENETICS, 2006, 173 (04) : 1969 - 1981
  • [9] The Impressionistic Landscape of Meiotic Recombination
    Lichten, Michael
    de Massy, Bernard
    [J]. CELL, 2011, 147 (02) : 267 - 270
  • [10] A Central Coupler for Recombination Initiation Linking Chromosome Architecture to S Phase Checkpoint
    Miyoshi, Tomoichiro
    Ito, Masaru
    Kugou, Kazuto
    Yamada, Shintaro
    Furuichi, Masaki
    Oda, Arisa
    Yamada, Takatomi
    Hirota, Kouji
    Masai, Hisao
    Ohta, Kunihiro
    [J]. MOLECULAR CELL, 2012, 47 (05) : 722 - 733
123