The COMPASS Subunit Spp1 Links Histone Methylation to Initiation of Meiotic Recombination

被引：156

作者：

Acquaviva, Laurent ^{[1
]}

Szekvoelgyi, Lorant ^{[2
,4
]}

Dichtl, Bernhard ^{[3
]}

Dichtl, Beatriz Solange ^{[3
]}

Saint Andre, Christophe de La Roche ^{[1
]}

Nicolas, Alain ^{[2
]}

Geli, Vincent ^{[1
]}

机构：

[1] Aix Marseille Univ, Inst Paoli Calmettes, Marseille Canc Res Ctr CRCM, INSERM,CNRS,U1068,UMR7258, F-13009 Marseille, France

[2] Univ Paris 06, CNRS, Inst Curie, Ctr Rech,UMR3244, F-75248 Paris, France

[3] Deakin Univ, Sch Life & Environm Sci, Ctr Cellular & Mol Biol, Melbourne, Vic 3125, Australia

[4] Univ Debrecen, Med & Hlth Sci Ctr, Dept Biophys & Cell Biol, H-4012 Debrecen, Hungary

来源：

SCIENCE | 2013年 / 339卷 / 6116期

基金：

瑞士国家科学基金会;

关键词：

STRAND BREAK FORMATION; H3; TRIMETHYLATION; GENE-EXPRESSION; SITES; PROMOTER; FEATURES; HOTSPOTS; COMPLEX; PRDM9; ONSET;

D O I：

10.1126/science.1225739

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions.

引用

页码：215 / 218

页数：4

共 24 条

[1] PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice [J].

Baudat, F. ;

Buard, J. ;

Grey, C. ;

Fledel-Alon, A. ;

Ober, C. ;

Przeworski, M. ;

Coop, G. ;

de Massy, B. .

SCIENCE, 2010, 327 (5967) :836-840

[2] Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation [J].

Blat, Y ;

Protacio, RU ;

Hunter, N ;

Kleckner, N .

CELL, 2002, 111 (06) :791-802

[3] Histone H3 lysine 4 trimethylation marks meiotic recombination initiation sites [J].

Borde, Valerie ;

Robine, Nicolas ;

Lin, Waka ;

Bonfils, Sandrine ;

Geli, Vincent ;

Nicolas, Alain .

EMBO JOURNAL, 2009, 28 (02) :99-111

[4] Protein interactions within the Set1 complex and their roles in the regulation of histone 3 lysine 4 methylation [J].

Dehe, Pierre-Marie ;

Dichtl, Bernhard ;

Schaft, Daniel ;

Roguev, Assen ;

Pamblanco, Merce ;

Lebrun, Regine ;

Rodriguez-Gil, Alfonso ;

Mkandawire, Msau ;

Landsberg, Katarina ;

Shevchenko, Anna ;

Shevchenko, Andrej ;

Rosaleny, Lorena E. ;

Tordera, Vicente ;

Chavez, Sebastian ;

Stewart, A. Francis ;

Geli, Vincent .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (46) :35404-35412

[5] Cyclin-dependent kinase directly regulates initiation of meiotic recombination [J].

Henderson, Kiersten A. ;

Kee, Kehkooi ;

Maleki, Shohreh ;

Santini, Paul A. ;

Keeney, Scott .

CELL, 2006, 125 (07) :1321-1332

[6]

Keeney Scott, 2008, V2, P81, DOI 10.1007/7050_2007_026

[7] Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6 [J].

Lardenois, Aurelie ;

Liu, Yuchen ;

Walther, Thomas ;

Chalmel, Frederic ;

Evrard, Bertrand ;

Granovskaia, Marina ;

Chu, Angela ;

Davis, Ronald W. ;

Steinmetz, Lars M. ;

Primig, Michael .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (03) :1058-1063

[8] Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation [J].

Li, Jing ;

Hooker, Gillian W. ;

Roeder, G. Shirleen .

GENETICS, 2006, 173 (04) :1969-1981

[9] The Impressionistic Landscape of Meiotic Recombination [J].

Lichten, Michael ;

de Massy, Bernard .

CELL, 2011, 147 (02) :267-270

[10] A Central Coupler for Recombination Initiation Linking Chromosome Architecture to S Phase Checkpoint [J].

Miyoshi, Tomoichiro ;

Ito, Masaru ;

Kugou, Kazuto ;

Yamada, Shintaro ;

Furuichi, Masaki ;

Oda, Arisa ;

Yamada, Takatomi ;

Hirota, Kouji ;

Masai, Hisao ;

Ohta, Kunihiro .

MOLECULAR CELL, 2012, 47 (05) :722-733

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