Molecular epidemiology of hepatitis B virus isolated from Bangladesh

被引:9
作者
Shaha, Modhusudon [1 ,2 ]
Hoque, Sheikh Ariful [3 ]
Rahman, Sabita Rezwana [1 ]
机构
[1] Natl Inst Biotechnol, Microbial Biotechnol Div, Dhaka 1349, Bangladesh
[2] Univ Dhaka, Dept Microbiol, Dhaka 1000, Bangladesh
[3] Univ Dhaka, Ctr Adv Res Sci, Cell & Tissue Culture Lab, Dhaka 1000, Bangladesh
来源
SPRINGERPLUS | 2016年 / 5卷
关键词
Hepatitis B virus; Genotypes; Mutations; Partial S gene; Bangladesh; S GENE; MUTATIONS; GENOTYPES; STRAINS; TRANSMISSION; RELATEDNESS; RESISTANCE; INFECTION;
D O I
10.1186/s40064-016-3174-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Hepatitis B virus (HBV) is highly contagious and causes liver diseases. Globally more than 350 million people are chronically infected and among them above 80 % are from developing countries like Bangladesh. Resistance to existing drugs and vaccines are common phenomenon due to mutations in HBsAg 'a' determinant. Due to lack of data about mutations and subtypes of HBV in Bangladesh, this study strongly demands to be documented. Here, we determined the genotypes and subtypes of HBV prevalent in Bangladesh, and their genomic mutations associated with vaccine and drug resistance. Results: Among 385 samples, a total of 54 (14 %) were found HBV positive, of which 19 samples were subjected to be sequenced. After bioinformatic analysis, we found Genotype D as predominant genotype (73.7 %) with subtypes ayw3 (64.3 %) and ayw(2) (35.7 %), followed by genotype A with subtype adw(2) (15.8 %), and then genotype C with subtype adr (10.5 %). A significant number of mutations (Thr118Val, Thr125Met, Thr126Ile, Pro127Thr, Ala128Val, Thr131Asn/Ser, Thr/Ser143Leu/Met) were found in 'a' determinant region which may admit resistance to the available vaccines and failure of HBsAg detection. Conclusions: This comprehensive study have clinical importance like disease diagnosis and treatment. It emphasizes HBV infected patients to do molecular diagnosis for choice of anti-viral drugs and effectiveness of vaccines for proper treatment.
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页数:7
相关论文
共 25 条
[1]  
Al-Mahtab M, 2008, HEPAT MON, V8, P147
[2]   Relationships within and between genotypes of hepatitis B virus at points across the genome: footprints of recombination in certain isolates [J].
Bowyer, SM ;
Sim, JGM .
JOURNAL OF GENERAL VIROLOGY, 2000, 81 :379-392
[3]   VACCINE-INDUCED ESCAPE MUTANT OF HEPATITIS-B VIRUS [J].
CARMAN, WF ;
ZANETTI, AR ;
KARAYIANNIS, P ;
WATERS, J ;
MANZILLO, G ;
TANZI, E ;
ZUCKERMAN, AJ ;
THOMAS, HC .
LANCET, 1990, 336 (8711) :325-329
[4]   Characterization of hepatitis B virus reverse transcriptase sequences in Chinese treatment naive patients [J].
Han, Yue ;
Huang, Li Hua ;
Liu, Chuan Miao ;
Yang, Shu ;
Li, Juan ;
Lin, Zhi Mei ;
Kong, Xiao Fei ;
Yu, De Min ;
Zhang, Dong Hua ;
Jin, Gen Di ;
Lu, Zhi Meng ;
Gong, Qi Ming ;
Zhang, Xin Xin .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2009, 24 (08) :1417-1423
[5]   Antiviral Resistance Mutations and Genotype-Associated Amino Acid Substitutions in Treatment-Naive Hepatitis B Virus-Infected Individuals from the Indian Subcontinent [J].
Ismail, A. M. ;
Samuel, P. ;
Eapen, C. E. ;
Kannangai, R. ;
Abraham, P. .
INTERVIROLOGY, 2012, 55 (01) :36-44
[6]   Clustal W and clustal X version 2.0 [J].
Larkin, M. A. ;
Blackshields, G. ;
Brown, N. P. ;
Chenna, R. ;
McGettigan, P. A. ;
McWilliam, H. ;
Valentin, F. ;
Wallace, I. M. ;
Wilm, A. ;
Lopez, R. ;
Thompson, J. D. ;
Gibson, T. J. ;
Higgins, D. G. .
BIOINFORMATICS, 2007, 23 (21) :2947-2948
[7]   Cellular and virological mechanisms of HBV drug resistance [J].
Locarnini, S ;
Mason, WS .
JOURNAL OF HEPATOLOGY, 2006, 44 (02) :422-431
[8]  
Mamun-Al-Mahtab, 2006, Indian J Gastroenterol, V25, P219
[9]  
Mast Eric E., 2005, Morbidity and Mortality Weekly Report, V54, P1
[10]   Analysis Tool Web Services from the EMBL-EBI [J].
McWilliam, Hamish ;
Li, Weizhong ;
Uludag, Mahmut ;
Squizzato, Silvano ;
Park, Young Mi ;
Buso, Nicola ;
Cowley, Andrew Peter ;
Lopez, Rodrigo .
NUCLEIC ACIDS RESEARCH, 2013, 41 (W1) :W597-W600