Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis

被引:152
作者
Masters, Thomas A. [1 ]
Pontes, Bruno [1 ]
Viasnoff, Virgile [1 ,2 ]
Li, You [1 ]
Gauthier, Nils C. [1 ]
机构
[1] Natl Univ Singapore, Mechanobiol Inst, Singapore 117411, Singapore
[2] Ecole Super Phys & Chim Ind Paristech, CNRS, F-75005 Paris, France
关键词
frustrated phagocytosis; immunology; phagocytic cell; Rho GTPases; traffic; FC-RECEPTOR; PHAGOSOME FORMATION; LEADING-EDGE; CELLS; MACROPHAGES; EXOCYTOSIS; SECRETION; PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE; COORDINATION; CONTRACTION;
D O I
10.1073/pnas.1301766110
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Phagocytes clear the body of undesirable particles such as infectious agents and debris. To extend pseudopods over the surface of targeted particles during engulfment, cells must change shape through extensive membrane and cytoskeleton remodeling. We observed that pseudopod extension occurred in two phases. In the first phase, pseudopods extended rapidly, with actin polymerization pushing the plasma membrane forward. The second phase occurred once the membrane area from preexisting reservoirs was depleted, leading to increased membrane tension. Increased tension directly altered the small Rho GTPase Rac1, 3'-phosphoinositide, and cytoskeletal organization. Furthermore, it activated exocytosis of vesicles containing GPI-anchored proteins, increasing membrane area and phagocytosis efficiency for large particles. We thus propose that, during phagocytosis, membrane remodeling, cytoskeletal organization, and biochemical signaling are orchestrated by the mechanical signal of membrane tension. These results put a simple mechanical signal at the heart of understanding immunological responses.
引用
收藏
页码:11875 / 11880
页数:6
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