In vitro bactericidal and TEM study of the interaction of Ag-modified titania with coliform bacteria

被引：6

作者：

Bano, Ishrat ^{[1
]}

Kumar, R. Vasant ^{[1
]}

Hameed, Abdul ^{[2
]}

机构：

[1] Univ Cambridge, Cambridge, England

[2] Quaid I Azam Univ, Islamabad, Pakistan

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH | 2013年 / 23卷 / 05期

关键词：

AgTiO2; coliform; E.coli; nanocomposite; TEM; PHOTOCATALYTIC ACTIVITY; TIO2; SILVER; NANOPARTICLES; DISINFECTION; OXIDATION; CHARGE;

D O I：

10.1080/09603123.2012.743115

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This research aimed to investigate in vitro photocatalytic bactericidal effect of Ag-TiO2 nanocomposite using Escherichia coli as a model organism. Highly dispersed, Ag-TiO2 nanocomposite is used with an average particle size of less than 20nm. Bactericidal analysis was carried out in Luria Bertani medium on solid agar plates with various illumination time and different concentrations of Ag-TiO2 nanocomposite. Transmission electron microscopic analysis of bacterial section was used to detect the effect of irradiation of Ag-TiO2 nanocomposite on the ultra structure of the bacterial cell in order to reveal possible cellular damage. The mechanism underlying the action of photoexcited Ag-TiO2 nanocomposite on E. coli cell membrane is also evaluated. The results confirmed that E. coli cells after the contact with Ag-TiO2 nanocomposite were damaged since they showed membrane disorganization. This causes the enhanced level of membrane permeability leading to the build-up of Ag-TiO2 nanocomposite in the bacterial membrane and also cellular internalization of these nanoparticles.

引用

页码：407 / 418

页数：12

共 27 条

[11] The effect of platinum and silver deposits in the photocatalytic oxidation of resorcinol [J].

Lam, Sai Wei ;

Chiang, Ken ;

Lim, Tuti M. ;

Amal, Rose ;

Low, Gary K-C. .

APPLIED CATALYSIS B-ENVIRONMENTAL, 2007, 72 (3-4) :363-372

[12] Synthesis of photocatalytic nanosized TiO2-Ag particles with sol-gel method using reduction agent [J].

Lee, MS ;

Hong, SS ;

Mohseni, M .

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 2005, 242 (1-2) :135-140

[13] A mechanism for enhanced photocatalytic activity of silver-loaded titanium dioxide [J].

Liu, SX ;

Qu, ZP ;

Han, XW ;

Sun, CL .

CATALYSIS TODAY, 2004, 93-5 :877-884

[14]

Lou H, 2010, PLOS ONE, V6

[15]

MATSUNAGA T, 1985, FEMS MICROBIOL LETT, V29, P211, DOI 10.1111/j.1574-6968.1985.tb00864.x

[16] Characterization of silver nanoparticles synthesized on titanium dioxide fine particles [J].

Nino-Martinez, N. ;

Martinez-Castanon, G. A. ;

Aragon-Pina, A. ;

Martinez-Gutierrez, F. ;

Martinez-Mendoza, J. R. ;

Ruiz, Facundo .

NANOTECHNOLOGY, 2008, 19 (06)

[17] PHOTOCATALYTICALLY-MEDIATED DISINFECTION OF WATER USING TIO2 AS A CATALYST AND SPORE-FORMING BACILLUS-PUMILUS AS A MODEL [J].

PHAM, HN ;

MCDOWELL, T ;

WILKINS, E .

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-ENVIRONMENTAL SCIENCE AND ENGINEERING & TOXIC AND HAZARDOUS SUBSTANCE CONTROL, 1995, 30 (03) :627-636

[18] CHARGE CARRIER TRAPPING AND RECOMBINATION DYNAMICS IN SMALL SEMICONDUCTOR PARTICLES [J].

ROTHENBERGER, G ;

MOSER, J ;

GRATZEL, M ;

SERPONE, N ;

SHARMA, DK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (26) :8054-8059

[19] Effect of deposition of Ag on TiO2 nanoparticles on the photodegradation of Reactive Yellow-17 [J].

Rupa, A. Valentine ;

Manikandan, D. ;

Divakar, D. ;

Sivakumar, T. .

JOURNAL OF HAZARDOUS MATERIALS, 2007, 147 (03) :906-913

[20] MODE OF PHOTOCATALYTIC BACTERICIDAL ACTION OF POWDERED SEMICONDUCTOR TIO2 ON MUTANS STREPTOCOCCI [J].

SAITO, T ;

IWASE, T ;

HORIE, J ;

MORIOKA, T .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 14 (04) :369-379

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