Genome-scale genetic engineering in Escherichia coli

被引:34
作者
Jeong, Jaehwan [1 ]
Cho, Namjin [1 ]
Jung, Daehee [1 ]
Bang, Duhee [1 ]
机构
[1] Yonsei Univ, Dept Chem, Seoul 120749, South Korea
基金
新加坡国家研究基金会;
关键词
Genome engineering; lambda Red recombination; Multiplex automated genome engineering; GRAM-NEGATIVE BACTERIA; SINGLE-STRANDED-DNA; HOMOLOGOUS RECOMBINATION; IN-VIVO; RECBCD ENZYME; BETA-PROTEIN; PHAGE-LAMBDA; BREAK-REPAIR; MISMATCH REPAIR; RECF PATHWAY;
D O I
10.1016/j.biotechadv.2013.04.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli as well as multiplex automated genome engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:804 / 810
页数:7
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