Prioritization of candidate genes in "QTL-hotspot" region for drought tolerance in chickpea (Cicer arietinum L.)

被引：117

作者：

Kale, Sandip M. ^{[1
]}

Jaganathan, Deepa ^{[1
,2
]}

Ruperao, Pradeep ^{[3
,4
,5
]}

Chen, Charles ^{[6
]}

Punna, Ramu ^{[7
]}

Kudapa, Himabindu ^{[1
]}

Thudi, Mahendar ^{[1
]}

Roorkiwal, Manish ^{[1
]}

Katta, Mohan A. V. S. K. ^{[1
]}

Doddamani, Dadakhalandar ^{[1
]}

Garg, Vanika ^{[1
]}

Kishor, P. B. Kavi ^{[2
]}

Gaur, Pooran M. ^{[1
]}

Nguyen, Henry T. ^{[8
,9
]}

Batley, Jacqueline ^{[3
,4
]}

Edwards, David ^{[3
,4
]}

Sutton, Tim ^{[10
,11
,12
]}

Varshney, Rajeev K. ^{[1
,3
,4
]}

机构：

[1] CEG, ICRISAT, Hyderabad 502324, Andhra Pradesh, India

[2] Osmania Univ, Dept Genet, Hyderabad 500007, Andhra Pradesh, India

[3] Univ Western Australia, Sch Plant Biol, Crawley 6009, Australia

[4] Univ Western Australia, Inst Agr, Crawley 6009, Australia

[5] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia

[6] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74074 USA

[7] Cornell Univ, Ithaca, NY 14853 USA

[8] Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA

[9] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA

[10] South Australian Res & Dev Inst, Adelaide, SA 5001, Australia

[11] Univ Adelaide, Adelaide, SA 5005, Australia

[12] Sch Agr, Adelaide, SA 5064, Australia

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

STRESS; RICE; MAIZE; LEAF; MAP; LINKAGE; SIZE;

D O I：

10.1038/srep15296

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A combination of two approaches, namely QTL analysis and gene enrichment analysis were used to identify candidate genes in the "QTL-hotspot" region for drought tolerance present on the Ca4 pseudomolecule in chickpea. In the first approach, a high-density bin map was developed using 53,223 single nucleotide polymorphisms (SNPs) identified in the recombinant inbred line (RIL) population of ICC 4958 (drought tolerant) and ICC 1882 (drought sensitive) cross. QTL analysis using recombination bins as markers along with the phenotyping data for 17 drought tolerance related traits obtained over 1-5 seasons and 1-5 locations split the "QTL-hotspot" region into two subregions namely "QTL-hotspot_a" (15 genes) and "QTL-hotspot_b" (11 genes). In the second approach, gene enrichment analysis using significant marker trait associations based on SNPs from the Ca4 pseudomolecule with the above mentioned phenotyping data, and the candidate genes from the refined "QTL-hotspot" region showed enrichment for 23 genes. Twelve genes were found common in both approaches. Functional validation using quantitative real-time PCR (qRT-PCR) indicated four promising candidate genes having functional implications on the effect of "QTL-hotspot" for drought tolerance in chickpea.

引用

页数：14

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