Chemical structure of a partially 3-O-methylated mannofucogalactan from edible mushroom Grifola frondosa

被引：20
|
作者：
Faria Oliveira, Gracy Kelly [1 ]
da Silva, Estefania Viano [1 ,2 ]
Ruthes, Andrea Caroline [3 ,4 ]
Liao, Luciano Morais [2 ]
Iacomini, Marcello [3 ]
Carbonero, Elaine R. [1 ]
机构：
[1] Univ Fed Goias, Dept Quim, BR-75704020 Catalao, Regional Catala, Brazil
[2] Univ Fed Goias, Inst Quim, Lab Ressonancia Magnet Nucl, Campus Samambaia, BR-74001970 Goiania, Go, Brazil
[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana, Brazil
[4] Univ Florida, GCREC, Dept Entomol & Nematol, 14625 Cty Rd 672, Wimauma, FL 33598 USA
来源：
CARBOHYDRATE POLYMERS | 2018年 / 187卷
关键词：
Medicinal mushroom; Grifola frondosa; Mannofucogalactan; Chemical structure; SOLUBLE NEUTRAL POLYSACCHARIDES; FRUIT BODIES; ANTITUMOR-ACTIVITY; AGARICUS-BISPORUS; MAITAKE MUSHROOM; MANNOGALACTAN; HETEROPOLYSACCHARIDE; HETEROGALACTAN; FUCOGALACTAN; ELUCIDATION;
D O I：
10.1016/j.carbpol.2018.01.080
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An unusual heteropolysaccharide was isolated from the fruiting bodies of the medicinal mushroom Grifola frondosa, via successive cold aqueous extraction, followed by fractionation through freeze-thawing, precipitation with Fehling solution and dialysis using a membrane with a size exclusion cut-off of 500 kDa. Its chemical structure was determined based on total acid hydrolysis, methylation analysis and NMR studies. The mannofucogalactan had a molar mass of 15.9 x 10(3) g mol(-1), which was determinate by HPSEC-MALLS. This heteropolymer showed to have a main chain of (1 -> 6)-linked alpha-D-Galp partially substituted at O-2 by 3-O-alpha-D-mannopyranosyl-alpha-L-fucopyranosyl groups and in a minor proportion with alpha-L-Fucp single-unit side chains. Moreover, the presence of 3-O-Me-Galp units could also be observed in the main chain of the G. frondosa mannofucogalactan.
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页码：110 / 117
页数：8
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