Chemical structure of a partially 3-O-methylated mannofucogalactan from edible mushroom Grifola frondosa

被引：20

作者：

Faria Oliveira, Gracy Kelly ^{[1
]}

da Silva, Estefania Viano ^{[1
,2
]}

Ruthes, Andrea Caroline ^{[3
,4
]}

Liao, Luciano Morais ^{[2
]}

Iacomini, Marcello ^{[3
]}

Carbonero, Elaine R. ^{[1
]}

机构：

[1] Univ Fed Goias, Dept Quim, BR-75704020 Catalao, Regional Catala, Brazil

[2] Univ Fed Goias, Inst Quim, Lab Ressonancia Magnet Nucl, Campus Samambaia, BR-74001970 Goiania, Go, Brazil

[3] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana, Brazil

[4] Univ Florida, GCREC, Dept Entomol & Nematol, 14625 Cty Rd 672, Wimauma, FL 33598 USA

来源：

CARBOHYDRATE POLYMERS | 2018年 / 187卷

关键词：

Medicinal mushroom; Grifola frondosa; Mannofucogalactan; Chemical structure; SOLUBLE NEUTRAL POLYSACCHARIDES; FRUIT BODIES; ANTITUMOR-ACTIVITY; AGARICUS-BISPORUS; MAITAKE MUSHROOM; MANNOGALACTAN; HETEROPOLYSACCHARIDE; HETEROGALACTAN; FUCOGALACTAN; ELUCIDATION;

D O I：

10.1016/j.carbpol.2018.01.080

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An unusual heteropolysaccharide was isolated from the fruiting bodies of the medicinal mushroom Grifola frondosa, via successive cold aqueous extraction, followed by fractionation through freeze-thawing, precipitation with Fehling solution and dialysis using a membrane with a size exclusion cut-off of 500 kDa. Its chemical structure was determined based on total acid hydrolysis, methylation analysis and NMR studies. The mannofucogalactan had a molar mass of 15.9 x 10(3) g mol(-1), which was determinate by HPSEC-MALLS. This heteropolymer showed to have a main chain of (1 -> 6)-linked alpha-D-Galp partially substituted at O-2 by 3-O-alpha-D-mannopyranosyl-alpha-L-fucopyranosyl groups and in a minor proportion with alpha-L-Fucp single-unit side chains. Moreover, the presence of 3-O-Me-Galp units could also be observed in the main chain of the G. frondosa mannofucogalactan.

引用

页码：110 / 117

页数：8