Detection of aryl hydrocarbon receptor agonists in human samples

被引：28

作者：

Rothhammer, Veit ^{[1
]}

Borucki, Davis M. ^{[1
]}

Kenison, Jessica E. ^{[1
]}

Hewson, Patrick ^{[1
]}

Wang, Zhongyan ^{[2
]}

Bakshi, Rohit ^{[1
]}

Sherr, David H. ^{[2
]}

Quintana, Francisco J. ^{[1
,3
]}

机构：

[1] Harvard Med Sch, Brigham & Womens Hosp, Ann Romney Ctr Neurol Dis, Boston, MA 02115 USA

[2] Boston Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA USA

[3] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

美国国家卫生研究院;

关键词：

MULTIPLE-SCLEROSIS; AH RECEPTOR; SERUM; ASSOCIATION; TRYPTOPHAN; LIGAND;

D O I：

10.1038/s41598-018-23323-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor with important functions in the immune response and cancer. AHR agonists are provided by the environment, the commensal flora and the metabolism. Considering AHR physiological functions, AHR agonists may have important effects on health and disease. Thus, the quantification of AHR agonists in biological samples is of scientific and clinical relevance. We compared different reporter systems for the detection of AHR agonists in serum samples of Multiple Sclerosis (MS) patients, and assessed the influence of transfection methods and cell lines in a reporter-based in vitro assay. While the use of stable or transient reporters did not influence the measurement of AHR agonistic activity, the species of the cell lines used in these reporter assays had important effects on the reporter readings. These observations suggest that cell-specific factors influence AHR activation and signaling. Thus, based on the reported species selectivity of AHR ligands and the cell species-of-origin effects that we describe in this manuscript, the use of human cell lines is encouraged for the analysis of AHR agonistic activity in human samples. These findings may be relevant for the analysis of AHR agonists in human samples in the context of inflammatory and neoplastic disorders.

引用

页数：8