Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine

被引：62

作者：

Henao-Escobar, W. ^{[1
]}

del Torno-de Roman, L. ^{[1
]}

Dominguez-Renedo, O. ^{[1
]}

Alonso-Lomillo, M. A. ^{[1
]}

Arcos-Martinez, M. J. ^{[1
]}

机构：

[1] Univ Burgos, Dept Analyt Chem, Fac Sci, Burgos 09001, Spain

来源：

FOOD CHEMISTRY | 2016年 / 190卷

关键词：

Dual enzymatic biosensor; Histamine; Putrescine; Screen-printed electrodes; Simultaneous determination; PRINTED CARBON ELECTRODES; BIOGENIC-AMINES; ACID DETERMINATION; DIAMINE OXIDASE; WINES; FOOD;

D O I：

10.1016/j.foodchem.2015.06.035

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A disposable electrodic system consisting of two working electrodes connected in array mode has been developed for the simultaneous determination of histamine (His) and putrescine (Put). Histamine deshydrogenase and putrescine oxidase enzymes were respectively immobilized by crosslinking on each working screen-printed electrode, both modified with tetrathiafulvalene. The dual system allowed the simultaneous amperometric determination of both species by measuring the oxidation current of the mediator in each working electrode. The effect of other potentially interfering biogenic amines was also evaluated. The capability of detection was of 8.1 +/- 0.7 for His and 10 +/- 0.6 mu M for Put. The precision in terms of relative standard deviation was of 3.5% and 6.7% for His and Put, respectively. The developed biosensor was successfully applied to the determination of His and Put in different food samples. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：818 / 823

页数：6

共 41 条

[1]

[Anonymous], 2000, ISO11843 2

[2]

[Anonymous], 1997, ISO11843 1

[3] Screen-printed biosensor based on the inhibition of the acetylcholinesterase activity for the determination of codeine [J].

Asturias-Arribas, Laura ;

Asuncion Alonso-Lomillo, M. ;

Dominguez-Renedo, Olga ;

Julia Arcos-Martinez, M. .

TALANTA, 2013, 111 :8-12

[4] CYP450 biosensors based on screen-printed carbon electrodes for the determination of cocaine [J].

Asturias-Arribas, Laura ;

Asuncion Alonso-Lomillo, M. ;

Dominguez-Renedo, Olga ;

Julia Arcos-Martinez, M. .

ANALYTICA CHIMICA ACTA, 2011, 685 (01) :15-20

[5] Disposable biosensors for determination of biogenic amines [J].

Asuncion Alonso-Lomillo, M. ;

Dominguez-Renedo, Olga ;

Matos, Patricia ;

Julia Arcos-Martinez, M. .

ANALYTICA CHIMICA ACTA, 2010, 665 (01) :26-31

[6] Spoilage Detection with Biogenic Amine Biosensors, Comparison of Different Enzyme Electrodes [J].

Boka, Beata ;

Adanyi, Nora ;

Virag, Diana ;

Sebela, Marek ;

Kiss, Attila .

ELECTROANALYSIS, 2012, 24 (01) :181-186

[7] Speciation of chromium using chronoamperometric biosensors based on screen-printed electrodes [J].

Calvo-Perez, Ana ;

Dominguez-Renedo, Olga ;

Alonso-Lomillo, MAsuncion ;

Arcos-Martinez, MJulia .

ANALYTICA CHIMICA ACTA, 2014, 833 :15-21

[8] Disposable amperometric biosensor for the determination of tyramine using plasma amino oxidase [J].

Calvo-Perez, Ana ;

Dominguez-Renedo, Olga ;

Asuncion Alonso-Lomillo, Maria ;

Julia Arcos-Martinez, Maria .

MICROCHIMICA ACTA, 2013, 180 (3-4) :253-259

[9] Development of screen-printed enzyme electrodes for the estimation of fish quality [J].

Chemnitius, GC ;

Bilitewski, U .

SENSORS AND ACTUATORS B-CHEMICAL, 1996, 32 (02) :107-113

[10] Gluconic acid determination in wine by electrochemical biosensing [J].

del Torno-de Roman, L. ;

Alonso-Lomillo, M. A. ;

Dominguez-Renedo, O. ;

Arcos-Martinez, M. J. .

SENSORS AND ACTUATORS B-CHEMICAL, 2013, 176 :858-862

← 1 2 3 4 5 →