Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry

被引：29
作者：
Fuh, MR [1 ]
Wu, TY [1 ]
Lin, TY [1 ]
机构：
[1] Soochow Univ, Dept Chem, Taipei 11102, Taiwan
来源：
TALANTA | 2006年 / 68卷 / 03期
关键词：
amphetamine; methamphetamine; ion-pair LC; LC-ES-MS;
D O I：
10.1016/j.talanta.2005.06.057
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method using a solid phase extraction (SPE) and ion-pair liquid chromatography-electrospray-tandem mass spectrometry (LC-ES-MS/MS) was developed for determination of amphetamine (Amp) and methamphetamine (mAmp) in urine samples. A reversed phase C-18 column was utilized for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. MS2 was employed for quantitative determination. In addition, d(8)-amphetamine and d(8)-methamphetamine were used as internal standards. An Oasis HLB SPE cartridge, which has hydrophilic and lipophilic functions, was utilized for sample pre-treatment. Recoveries ranging from 97.3 to 102.1% were measured. Good linear ranges, 5-500ng/ml, for Amp and mAmp were determined. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, was approximately 1ng/ml. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：987 / 991
页数：5
相关论文
共 24 条
[1]   Analysis of illicit drugs by nonaqueous capillary electrophoresis and electrochemical detection [J].
Backofen, U ;
Matysik, FM ;
Hoffmann, W ;
Lunte, CE .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2000, 367 (04) :359-363
[2]   Determination of amphetamine in rat brain by in vivo microdialysis and ion-pairing liquid chromatography with electrospray tandem mass spectrometry [J].
Fuh, MR ;
Haung, CH ;
Wu, TY ;
Lin, SL ;
Pan, WHT .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (15) :1711-1714
[3]   Simultaneous determination of free-form amphetamine in rat's blood and brain by in-vivo microdialysis and liquid chromatography with fluorescence detection [J].
Fuh, MR ;
Lin, HT ;
Pan, WHT ;
Lin, FR .
TALANTA, 2002, 58 (06) :1357-1363
[4]   Quantitative determination of sulfonamide in meat by solid-phase extraction and capillary electrophoresis [J].
Fuh, MRS ;
Chu, SY .
ANALYTICA CHIMICA ACTA, 2003, 499 (1-2) :215-221
[5]  
Harris D. C., 2020, QUANTITATIVE CHEM AN
[6]   QUANTITATION OF METHAMPHETAMINE AND AMPHETAMINE IN URINE BY CAPILLARY GC MS .1. ADVANTAGES OF TRICHLOROACETYL DERIVATIZATION [J].
HORNBECK, CL ;
CZARNY, RJ .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1989, 13 (03) :144-149
[7]  
Lee MS, 1999, MASS SPECTROM REV, V18, P187, DOI 10.1002/(SICI)1098-2787(1999)18:3/4<187::AID-MAS2>3.0.CO
[8]  
2-K
[9]   Chemical derivatization and the selection of deuterated internal standard for quantitative determination-methamphetamine example [J].
Lin, DL ;
Chang, WT ;
Kuo, TL ;
Liu, RH .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (04) :275-280
[10]   Determination of paramethoxyamphetamine and other amphetamine-related designer drugs by liquid chromatography/sonic spray ionization mass spectrometry [J].
Mortier, KA ;
Dams, R ;
Lambert, WE ;
De Letter, EA ;
Van Calenbergh, S ;
De Leenheer, AP .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (09) :865-870
123