An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display

被引：14

作者：

Kumachi, Shigefumi ^{[1
]}

Husimi, Yuzuru ^{[2
]}

Nemoto, Naoto ^{[1
]}

机构：

[1] Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, 255 Shimo Okubo, Saitama 3388570, Japan

[2] SOKENDAI, Hayama, Kanagawa 2400193, Japan

来源：

ACS OMEGA | 2016年 / 1卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

RANDOM-SEQUENCE PROTEINS; PUROMYCIN-LINKER; WORLD; KINDS; LIFE;

D O I：

10.1021/acsomega.6b00015

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

RNA-protein interactions have a central role in the living world. In this article, we examined whether primitive peptides (30 residues) consisting of four types of amino acid (Gly, Ala, Asp, and Val) could interact with tRNA as a model of primitive RNAs in the RNA world. By in vitro selection of binding peptides using the cDNA display method, a characteristic peptide was selected from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Interestingly, the selected peptide bound to a single-stranded region including a loop structure of an RNA molecule with some sequence specificity.

引用

页码：52 / 57

页数：6

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