FTIR analysis of molecular composition changes in hazel pollen from unpolluted and urbanized areas

被引:34
作者
Depciuch, J. [1 ]
Kasprzyk, I. [2 ]
Sadik, O. [3 ]
Parlinska-Wojtan, M. [1 ]
机构
[1] Polish Acad Sci, Inst Nucl Phys, PL-31342 Krakow, Poland
[2] Univ Rzeszow, Fac Biol & Agr, Dept Environm Biol, Cwiklinskiej 1a, PL-35601 Rzeszow, Poland
[3] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
关键词
FTIR; Hazel; Pollen; Allergy; Air pollution; SEM; AIR-POLLUTION; AMIDE-I; CORYLUS; SPECTROSCOPY; ASTHMA; PREVALENCE; MUTATIONS; ALLERGENS; SPECTRA; CLIMATE;
D O I
10.1007/s10453-016-9445-3
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study, the effect of urbanization and environmental pollution on qualitative (structural) and quantitative changes of the Corylus avellana (hazel) pollen was investigated using scanning electron microscopy, Fourier Transform Infrared (FTIR) spectroscopy and curve-fitting analysis of amide I profile. The obtained spectroscopic results show significant variations in the fraction of proteins in the hazel pollen, which probably depend on various degrees of anthropopression. Our results suggest that alterations in the chemical composition of pollen, induced by urbanization and air pollutants, may intensify the allergenic potential and may cause the increase in the incidence of allergies in people. Mutations in nucleic acids are accompanied by a number of molecular changes leading to the formation of allergenic proteins. It seems that the type of habitat, where the pollen grew, affects the individual differentiation. Indeed, it was found that in the site exhibiting low pollution, the hazel pollen contain a lower amount of proteins than to the ones from a site with high anthropopression. Hence, FTIR spectroscopy and curve-fitting analysis of amide I profile can be successfully applied as tools for identifying quantitative and qualitative changes of proteins in hazel pollen. Anthropogenic factors such as air pollution and urbanization lead to changes in structure and chemical composition of hazel pollen. Fourier Transform Infrared spectroscopy (FTIR) and Gaussian analysis showed structural changes in hazel pollen collected from sites with different absorbance values of individual chemical functional groups and changes in the secondary structure of proteins of the pollen. [GRAPHICS] .
引用
收藏
页码:1 / 12
页数:12
相关论文
共 50 条
[11]   Allergenic pollen and pollen allergy in Europe [J].
D'Amato, G. ;
Cecchi, L. ;
Bonini, S. ;
Nunes, C. ;
Annesi-Maesano, I. ;
Behrendt, H. ;
Liccardi, G. ;
Popov, T. ;
van Cauwenberge, P. .
ALLERGY, 2007, 62 (09) :976-990
[12]  
D'Amato G, 2000, CLIN EXP ALLERGY, V30, P628
[13]   Pollen discrimination and classification by Fourier transform infrared (FT-IR) microspectroscopy and machine learning [J].
Dell'Anna, R. ;
Lazzeri, P. ;
Frisanco, M. ;
Monti, F. ;
Campeggi, F. Malvezzi ;
Gottardini, E. ;
Bersani, M. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 394 (05) :1443-1452
[14]   FTIR spectroscopic characterization of irradiated hazelnut (Corylus avellana L.) [J].
Dogan, Ayca ;
Siyakus, Galip ;
Severcan, Feride .
FOOD CHEMISTRY, 2007, 100 (03) :1106-1114
[15]   ANNUAL VARIATIONS IN GRASS-POLLEN SEASONS IN LONDON 1961-1990 - TRENDS AND FORECAST MODELS [J].
EMBERLIN, J ;
SAVAGE, M ;
JONES, S .
CLINICAL AND EXPERIMENTAL ALLERGY, 1993, 23 (11) :911-918
[16]   Changes in the pollen seasons of the early flowering trees Alnus spp. and Corylus spp. in Worcester, United Kingdom, 1996-2005 [J].
Emberlin, Jean ;
Smith, Matt ;
Close, Rebecca ;
Adams-Groom, Beverley .
INTERNATIONAL JOURNAL OF BIOMETEOROLOGY, 2007, 51 (03) :181-191
[17]   WIDE-BAND DETECTOR FOR MICRO-MICROAMPERE LOW-ENERGY ELECTRON CURRENTS [J].
EVERHART, TE ;
THORNLEY, RFM .
JOURNAL OF SCIENTIFIC INSTRUMENTS, 1960, 37 (07) :246-248
[18]   Trends in prevalence of allergic rhinitis and correlation with pollen counts in Switzerland [J].
Frei, Thomas ;
Gassner, Ewald .
INTERNATIONAL JOURNAL OF BIOMETEOROLOGY, 2008, 52 (08) :841-847
[19]   A comprehensive catalogue of somatic mutations in cancer genomes [J].
Friedberg, Errol C. .
DNA REPAIR, 2010, 9 (04) :468-469
[20]   Use of Fourier transform infrared (FT-IR) spectroscopy as a tool for pollen identification [J].
Gottardini, Elena ;
Rossi, Stefano ;
Cristofolini, Fabiana ;
Benedetti, Luca .
AEROBIOLOGIA, 2007, 23 (03) :211-219