Modulatory Effect of 1,25-Dihydroxyvitamin D3 on IL1β-Induced RANKL, OPG, TNFα, and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A

被引:35
作者
Feng, Xiaoke [1 ,2 ]
Lv, Chengyin [1 ]
Wang, Fang [3 ]
Gan, Ke [1 ,4 ]
Zhang, Miaojia [1 ]
Tan, Wenfeng [1 ]
机构
[1] Nanjing Med Univ, Dept Rheumatol, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Tradit Chinese Med, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Dept Cardiol, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China
[4] Nanjing Tradit Chinese Med Univ, Dept Internal Med Tradit Chinese Med, Nanjing 210029, Jiangsu, Peoples R China
来源
CLINICAL & DEVELOPMENTAL IMMUNOLOGY | 2013年
基金
中国国家自然科学基金;
关键词
FIBROBLAST-LIKE SYNOVIOCYTES; VITAMIN-D; RECEPTOR ACTIVATOR; DISEASE-ACTIVITY; IMMUNE-SYSTEM; BONE LOSS; ARTHRITIS; LIGAND; CELLS; OSTEOCLASTOGENESIS;
D O I
10.1155/2013/160123
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Receptor activator of nuclear factor kappa B ligand (RANKL) plays a crucial role in the bone erosion of rheumatoid arthritis (RA) by prompting osteoclastogenesis. Considering that 1,25(OH)(2)D-3 has been suggested as a potent inducer of RANKL expression, it should clarify whether vitamin D supplement could result in RANKL overexpression and thereby facilitate excessive osteoclastogenesis and bone resorption in RA. Here, we investigated modulatory effect of 1,25(OH)(2)D-3 on the expression of RANKL and its decoy receptor osteoprotegerin (OPG) in an inflammatory condition of human rheumatoid synoviocyte MH7A. MH7A cells were stimulated with IL1 beta and then treated with different concentrations of 1,25(OH)(2)D-3 for 48 h. A significantly elevated OPG/RANKL ratio and markedly decreased levels of IL-6 and TNF beta mRNA expression in cells and IL-6 protein in supernatants were observed in IL1 beta-induced MH7A in the presence of 1,25(OH)(2)D-3 compared with those in the absence of it. Osteoclast formation was obviously decreased when RAW264.7 cells were treated with both 1,25(OH)(2)D-3 and IL1 beta. In summary, although it has a biological function to induce RANKL expression, 1,25(OH)(2)D-3 could upregulate OPG/RANKL ratio and mediate anti-inflammatory action in an inflammatory milieu of synoviocyte, contributing to the inhibition of inflammation-induced osteoclastogenesis in RA.
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页数:8
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