Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm

被引：43
作者：
Itabashi, Etsuko [1 ]
Kazama, Tomohiko [1 ]
Toriyama, Kinya [1 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Lab Environm Biotechnol, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
PLANT CELL REPORTS | 2009年 / 28卷 / 02期
关键词：
Cytoplasmic male sterility; Fertility restorer gene; Oryza sativa L; REPEAT-CONTAINING GENE; RESTORES FERTILITY; PPR MOTIF; PROTEIN; MITOCHONDRIAL; ENCODES; CLONING; RADISH; RF-1; ATP6;
D O I：
10.1007/s00299-008-0625-7
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Rice with LD-type cytoplasmic male sterility (CMS) possesses the cytoplasm of 'Lead Rice' and its fertility is recovered by a nuclear fertility restorer gene Rf1. Rf1 promotes processing of a CMS-associated mitochondrial RNA of atp6-orf79, which consists of atp6 and orf79, in BT-CMS with the cytoplasm of 'Chinsurah Boro II'. In this study, we found that LD-cytoplasm contained a sequence variant of orf79 downstream of atp6. Northern blot analysis showed that atp6-orf79 RNA of LD-cytoplasm was co-transcribed and was processed in the presence of Rf1 in the same manner as in BT-cytoplasm. Western blot analysis showed that the ORF79 peptide did not accumulate in an LD-CMS line, while ORF79 accumulated in a BT-CMS line and was diminished by Rf1. These results suggest that accumulation of ORF79 is not the cause of CMS in LD-cytoplasm and the mechanism of male-sterility induction/fertility restoration in LD-CMS is different from that in BT-CMS.
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页码：233 / 239
页数：7
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