Vaccination of mice with a modified Vaccinia Ankara (MVA) virus expressing the African horse sickness virus (AHSV) capsid protein VP2 induces virus neutralising antibodies that confer protection against AHSV upon passive immunisation

被引:25
作者
Calvo-Pinilla, Eva [1 ]
de la Poza, Francisco [2 ]
Gubbins, Simon [1 ]
Mertens, Peter Paul Clement [1 ]
Ortego, Javier [2 ]
Castillo-Olivares, Javier [1 ]
机构
[1] Pirbright Inst, Woking, Surrey, England
[2] CISA INIA, Ctr Invest Sanidad Anim, Madrid, Spain
基金
英国生物技术与生命科学研究理事会;
关键词
African horse sickness; Vaccinia; MVA-VP2; Protection; Humoral response; Passive immunisation; RECOMBINANT CANARYPOX VIRUS; IMMUNE-RESPONSES; IDENTIFICATION; GENES;
D O I
10.1016/j.virusres.2013.12.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In previous studies we showed that a recombinant Modified Vaccinia Ankara (MVA) virus expressing the protein VP2 of AHSV serotype 4 (MVA-VP2) induced virus neutralising antibodies in horses and protected interferon alpha receptor gene knock-out mice (IFNAR-/-) against challenge. We continued these studies and determined, in the IFNAR-/- mouse model, whether the antibody responses induced by MVA-VP2 vaccination play a key role in protection against AHSV. Thus, groups of mice were vaccinated with wild type MVA (MVA-wt) or MVA-VP2 and the antisera from these mice were used in a passive immunisation experiment. Donor antisera from (a) MVA-wt; (b) MVA-VP2 vaccinated; or (c) MVA-VP2 vaccinated and AHSV infected mice, were transferred to AHSV non-immune recipient mice. The recipients were challenged with virulent AHSV together with MVA-VP2 vaccinated and MVA-wt vaccinated control animals and the levels of protection against AHSV-4 were compared between all these groups. The results showed that following AHSV challenge, mice that were passively immunised with MVA-VP2 vaccinated antisera were highly protected against AHSV disease and had lower levels of viraemia than recipients of MVA-wt antisera. Our study indicates that MVA-VP2 vaccination induces a highly protective humoral immune response against AHSV. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:23 / 30
页数:8
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