Characteristics and immune-enhancing activity of pectic polysaccharides from sweet cherry (Prunus avium)

被引：82
作者：
Cao, Jinping [1 ,2 ]
Tang, Dandan [1 ]
Wang, Yue [1 ]
Li, Xian [1 ]
Hong, Li [2 ]
Sun, Chongde [1 ]
机构：
[1] Zhejiang Univ, State Agr Minist Lab Hort Plant Growth Dev & Qual, Lab Fruit Qual Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
[2] Taizhou Acad Agr Sci, Linhai 317000, Peoples R China
来源：
FOOD CHEMISTRY | 2018年 / 254卷
基金：
中国国家自然科学基金;
关键词：
Prunus avium; Polysaccharides; Characteristics; Immune-enhancing activity; WATER-SOLUBLE POLYSACCHARIDE; CELL-WALL POLYSACCHARIDES; IMMUNOSTIMULATORY POLYSACCHARIDES; FUNCTIONAL-PROPERTIES; CITRUS PECTIN; DIETARY FIBER; IN-VITRO; FRUIT; ACTIVATION; EXTRACTS;
D O I：
10.1016/j.foodchem.2018.01.145
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Two water soluble polysaccharides components PAPS-1 and PAPS-2 with homogeneously distributed molecular weight were obtained from Prunus avium. PAPS-1 and PAPS-2 contained GalA: Ara: Gal: Rha: GluA: Glu in 49.38: 32.39: 10.68: 4.66: 1.94: 0.48 and 77.18: 14.91: 3.39: 3.46: 0.93: 0.19M ratios respectively, as well as trace amount of mannose and fucose. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR) and methylation analysis indicated that both fractions were type I rhamnogalacturonan (RG-I) pectic polysaccharides with glycan side chains constituted mainly of arabinose with minor amount of galactose. Galacturonic acid methylation and sugar acetylation was found in both PAPS-1 and PAPS-2. Both PAPS-1 and PAPS-2 significantly induced the NO release from RAW264.7 cells and the expression of several immune-related molecular (TNF alpha, IL6, IL10, GCSF, iNOS, COX-2) was induced in RAW264.7 cells.
引用
收藏
页码：47 / 54
页数：8
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