Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

被引：17

作者：

Calcagno, Danielle Queiroz ^{[1
,2
]}

Takeno, Sylvia Santomi ^{[1
,3
]}

Gigek, Carolina Oliveira ^{[1
,4
]}

Leal, Mariana Ferreira ^{[1
,5
]}

Wisnieski, Fernanda ^{[1
]}

Chen, Elizabeth Suchi ^{[1
]}

Thomaz Araujo, Taissa Maira ^{[2
]}

Lima, Eleonidas Moura ^{[3
]}

Melaragno, Maria Isabel ^{[1
]}

Demachki, Samia ^{[2
]}

Assumpcao, Paulo Pimentel ^{[2
]}

Burbano, Rommel Rodriguez ^{[2
]}

Smith, Marilia Cardoso ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04021001 Sao Paulo, SP, Brazil

[2] Hosp Univ Joao Barros Barreto, Nucleo Pesquisas Oncol, Av Mundurucus 4487,1 Piso Unacon, BR-66073000 Belem, PA, Brazil

[3] Univ Fed Paraiba, Dept Biol Mol, BR-58051900 Joao Pessoa, Paraiba, Brazil

[4] Univ Fed Sao Paulo, Disciplina Gastroenterol Cirurg, BR-04021001 Sao Paulo, SP, Brazil

[5] Univ Fed Sao Paulo, Dept Ortopedia & Traumatol, BR-04021001 Sao Paulo, SP, Brazil

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2016年 / 22卷 / 43期

基金：

巴西圣保罗研究基金会;

关键词：

IL11RA; Gastric cancer; Genomic profiling; MELK; 9p13.3; INTERLEUKIN-11; RECEPTOR-ALPHA; CONVENTIONAL CYTOGENETIC CHARACTERIZATION; COPY NUMBER ALTERATIONS; C-MYC AMPLIFICATION; HUMAN BREAST-CANCER; GENETIC ALTERATIONS; PROSTATE-CANCER; INCREASED EXPRESSION; THERAPEUTIC TARGET; NORTHERN BRAZIL;

D O I：

10.3748/wjg.v22.i43.9506

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM To identify common copy number alterations on gastric cancer cell lines. METHODS Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha (IL11RA) and maternal embryonic leucine zipper kinase (MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11RA and MELK was observed in 19.1% (13/68) and 55.9% (38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.

引用

页码：9506 / 9514

页数：9

共 57 条

[1]

Burbano RR, 2006, ANTICANCER RES, V26, P2909

[2] Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma [J].

Calcagno, Danielle Queiroz ;

Leal, Mariana Ferreira ;

Seabra, Aline Damaceno ;

Khayat, Andre Salim ;

Chen, Elizabeth Suchi ;

Demachki, Samia ;

Assumpcao, Paulo Pimentel ;

Girao Faria, Mario Henrique ;

Barem Rabenhorst, Silvia Helena ;

Pitombeira Ferreira, Marcia Valeria ;

Cardoso Smith, Marilia de Arruda ;

Burbano, Rommel Rodriguez .

WORLD JOURNAL OF GASTROENTEROLOGY, 2006, 12 (38) :6207-6211

[3] MYC, FBXW7 and TP53 copy number variation and expression in Gastric Cancer [J].

Calcagno, Danielle Queiroz ;

Freitas, Vanessa Morais ;

Leal, Mariana Ferreira ;

Teixeira de Souza, Carolina Rosal ;

Demachki, Samia ;

Montenegro, Raquel ;

Assumpcao, Paulo Pimentel ;

Khayat, Andre Salim ;

Cardoso Smith, Marilia de Arruda ;

Ribeiro dos Santos, Andrea Kely Campos ;

Burbano, Rommel Rodriguez .

BMC GASTROENTEROLOGY, 2013, 13

[4]

Calcagno DQ, 2009, ANTICANCER RES, V29, P2479

[5]

Calcagno DQ, 2005, ANTICANCER RES, V25, P4069

[6] Increased expression of the interleukin-11 receptor and evidence of STAT3 activation in prostate carcinoma [J].

Campbell, CL ;

Jiang, Z ;

Savarese, DMF ;

Savarese, TM .

AMERICAN JOURNAL OF PATHOLOGY, 2001, 158 (01) :25-32

[7] MELK inhibitor, novel molecular targeted therapeutics for human cancer stem cells [J].

Chung, Suyoun ;

Nakamura, Yusuke .

CELL CYCLE, 2013, 12 (11) :1655-1656

[8]

Dos Santos SEB, 1999, AM J PHYS ANTHROPOL, V109, P175, DOI 10.1002/(SICI)1096-8644(199906)109:2<175::AID-AJPA3>3.0.CO

[9]

2-#

[10] Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway [J].

Du, Tao ;

Qu, Ying ;

Li, Jianfang ;

Li, Hao ;

Su, Liping ;

Zhou, Quan ;

Yan, Min ;

Li, Chen ;

Zhu, Zhenggang ;

Liu, Bingya .

MOLECULAR CANCER, 2014, 13

← 1 2 3 4 5 6 →