Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation

被引：97

作者：

Winchell, Jonas M. ^{[1
]}

Thurman, Kathleen A. ^{[1
]}

Mitchell, Stephanie L. ^{[1
]}

Thacker, W. Lanier ^{[1
]}

Fields, Barry S. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Resp Dis Branch, Atlanta, GA 30333 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 09期

关键词：

D O I：

10.1128/JCM.00440-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.

引用

页码：3116 / 3118

页数：3

共 18 条

[1] ALEXANDER NE, 2007, 45 INF DIS SOC AM AN
[2] The atypical pneumonias: clinical diagnosis and importance
Cunha, BA
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 : 12 - 24
[3] Laboratory diagnosis of Mycoplasma pneumoniae infection
Daxboeck, F
Krause, R
Wenisch, C
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (04) : 263 - 273
[4] Molecular detection of Mycoplasma pneumoniae in adults with community-acquired pneumonia requiring hospitalization
Dorigo-Zetsma, JW
Verkooyen, RP
van Helden, HP
van der Nat, H
van den Bosch, JM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) : 1184 - 1186
[5] Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR
Gullsby, Karolina
Storm, Martin
Bondeson, Kare
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (02) : 727 - 731
[6] IDENTIFICATION OF A POSSIBLE CYTADHERENCE REGULATORY LOCUS IN MYCOPLASMA-PNEUMONIAE
HEDREYDA, CT
KRAUSE, DC
[J]. INFECTION AND IMMUNITY, 1995, 63 (09) : 3479 - 3483
[7] Azithromycin prophylaxis during a hospital outbreak of Mycoplasma pneumoniae pneumonia
Hyde, TB
Gilbert, M
Schwartz, SB
Zell, ER
Watt, JP
Thacker, WL
Talkington, DF
Besser, RE
[J]. JOURNAL OF INFECTIOUS DISEASES, 2001, 183 (06) : 907 - 912
[8] ADP-ribosylating and vacuolating cytotoxin of Mycoplasma pneumoniae represents unique virulence determinant among bacterial pathogens
Kannan, TR
Baseman, JB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (17) : 6724 - 6729
[9] Enhanced control of an outbreak of Mycoplasma pneumoniae pneumonia with azithromycin prophylaxis
Klausner, JD
Passaro, D
Rosenberg, J
Thacker, WL
Talkington, DF
Werner, SB
Vugia, DJ
[J]. JOURNAL OF INFECTIOUS DISEASES, 1998, 177 (01) : 161 - 166
[10] Identification of risk factors for infection in an outbreak of Mycoplasma pneumoniae respiratory tract disease
Klement, Eyal
Talkington, Deborah F.
Wasserzug, Oshri
Kayouf, Raid
Davidovitch, Nadav
Dumke, Roger
Bar-Zeev, Yael
Ron, Merav
Boxman, Jonathan
Thacker, W. Lanier
Wolf, Dana
Lazarovich, Tsilia
Shemer-Avni, Yonat
Glikman, Daniel
Jacobs, Enno
Grotto, Itamar
Block, Colin
Nir-Paz, Ran
[J]. CLINICAL INFECTIOUS DISEASES, 2006, 43 (10) : 1239 - 1245

← 1 2 →