Real-time RT-PCR Assay for the detection of Tahyna Virus

被引：12

作者：

Li Hao ^{[1
]}

Cao Yu Xi ^{[1
]}

He Xiao Xia ^{[1
]}

Fu Shi Hong ^{[1
]}

Lyu Zhi ^{[1
]}

He Ying ^{[1
]}

Gao Xiao Yang ^{[1
]}

Liang Guo Dong ^{[1
]}

Wang Huang Yu ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2015年 / 28卷 / 05期

关键词：

MOSQUITO-BORNE ARBOVIRUSES; QINGHAI-TIBET PLATEAU; CHINA; INFECTION;

D O I：

10.3967/bes2015.052

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance.

引用

页码：374 / 377

页数：4

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