Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag

被引：121

作者：

Hudak, Jason E. ^{[1
]}

Barfield, Robyn M. ^{[2
]}

de Hart, Gregory W. ^{[2
]}

Grob, Patricia ^{[3
]}

Nogales, Eva ^{[3
]}

Bertozzi, Carolyn R. ^{[1
]}

Rabuka, David ^{[2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Redwood Biosci Inc, Emeryville, CA 94608 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 17期

关键词：

antibodies; bioorganic chemistry; click chemistry; oximes; proteins; RECOMBINANT PROTEINS; ANTIBODY; THERAPY; CONJUGATION; GENERATION; MECHANISM; GLYCANS;

D O I：

10.1002/anie.201108130

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Clicking into place: Cu-free click chemistry is combined with the aldehyde tag protein modification strategy to produce heterobifunctional protein fusions. This method relies on linkers that utilize orthogonal triazole and oxime chemistries (see scheme) to expand on conjugation with the fGly residue and enables site-specific protein conjugation to full-length human antibodies. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4161 / 4165

页数：5

共 56 条

[1] Antibody-enzyme fusion proteins for cancer therapy [J].

Andrady, Carima ;

Sharma, Surinder K. ;

Chester, Kerry A. .

IMMUNOTHERAPY, 2011, 3 (02) :193-211

[2] A recombinant human glucagon-like peptide (GLP)-1-albumin protein (Albugon) mimics peptidergic activation of GLP-1 receptor-dependent pathways coupled with satiety, gastrointestinal motility, and glucose homeostasis [J].

Baggio, LL ;

Huang, QL ;

Brown, TJ ;

Drucker, DJ .

DIABETES, 2004, 53 (09) :2492-2500

[3] Strategies and challenges for the next generation of therapeutic antibodies [J].

Beck, Alain ;

Wurch, Thierry ;

Bailly, Christian ;

Corvaia, Nathalie .

NATURE REVIEWS IMMUNOLOGY, 2010, 10 (05) :345-352

[4] Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein-Protein Click Conjugation [J].

Bundy, Bradley C. ;

Swartz, James R. .

BIOCONJUGATE CHEMISTRY, 2010, 21 (02) :255-263

[5] Design of next-generation protein therapeutics [J].

Caravella, Justin ;

Lugovskoy, Alexey .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (04) :520-528

[6] Introducing genetically encoded aldehydes into proteins [J].

Carrico, Isaac S. ;

Carlson, Brian L. ;

Bertozzi, Carolyn R. .

NATURE CHEMICAL BIOLOGY, 2007, 3 (06) :321-322

[7] Introduction to current and future protein therapeutics: A protein engineering perspective [J].

Carter, Paul J. .

EXPERIMENTAL CELL RESEARCH, 2011, 317 (09) :1261-1269

[8] A "Tag-and-Modify" Approach to Site-Selective Protein Modification [J].

Chalker, Justin M. ;

Bernardes, Goncalo J. L. ;

Davis, Benjamin G. .

ACCOUNTS OF CHEMICAL RESEARCH, 2011, 44 (09) :730-741

[9] Bispecific antibodies for cancer therapy The light at the end of the tunnel? [J].

Chames, Patrick ;

Baty, Daniel .

MABS, 2009, 1 (06) :539-547

[10]

Chamow S.M., 1999, Antibody Fusion Proteins

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