Encapsulation of flavodoxin in reverse micelles

被引:10
作者
Andrade, S
Kamenskaya, EO
Levashov, AV
Moura, JJG
机构
[1] UNIV NOVA LISBOA,FAC CIENCIAS & TECNOL,CTR QUIM FINE & BIOTECHNOL,DEPT QUIM,P-2825 MONTE DE CAPARICA,PORTUGAL
[2] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,DIV CHEM ENZYMOL,MOSCOW 119899,RUSSIA
关键词
D O I
10.1006/bbrc.1997.6525
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The regulation of the properties of Desulfovibrio gigas flavodoxin in AOT/water/iso-octane micellar system was studied. UV-visible spectroscopic studies have shown that photoreduction of flavodoxin in the presence of EDTA leads to hydroquinone formation through the intermediate semiquinone. The [free FMN] - [bound to flavodoxin PMN] equilibrium (and hence, the amount of apoprotein) depends on redox state of FMN and on hydration degree which controls the micellar size. Thus, a new method of reversible cofactor removing under mild conditions (at low hydration degree of micelles) is suggested, accompained by isolation of apo-form of the protein. (C) 1997 Academic Press.
引用
收藏
页码:651 / 654
页数:4
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