Isolation and characterization of ten polymorphic microsatellite loci in Ixodes arboricola, and cross-amplification in three other Ixodes species

被引：6
作者：
Van Houtte, N. [1 ]
Van Oosten, A. R. [1 ]
Jordaens, K. [1 ,2 ]
Matthysen, E. [1 ]
Backeljau, T. [1 ,3 ,4 ]
Heylen, D. J. A. [1 ]
机构：
[1] Univ Antwerp, Evolutionary Ecol Grp, B-2020 Antwerp, Belgium
[2] Royal Museum Cent Africa, JEMU, B-3080 Tervuren, Belgium
[3] Royal Belgian Inst Nat Sci, Dept Invertebrates, B-1000 Brussels, Belgium
[4] Royal Belgian Inst Nat Sci, JEMU, B-1000 Brussels, Belgium
来源：
EXPERIMENTAL AND APPLIED ACAROLOGY | 2013年 / 61卷 / 03期
关键词：
Microsatellite; Ixodes arboricola; Tree hole tick; Host race formation; LYME BORRELIOSIS; HOST RACES; PARASITE POPULATIONS; GENETIC-STRUCTURE; MIGRATORY BIRDS; EUROPEAN VECTOR; TICK; RICINUS; DETACHMENT; EVOLUTION;
D O I：
10.1007/s10493-013-9702-x
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 11-18 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from Hardy-Weinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in a parts per thousand yen4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners.
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页码：327 / 336
页数：10
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