Stellate Cells via JAK2-STAT3 Pathway to Protect against Hepatic Fibrosis

被引:2
|
作者
Wei, Yanfei [1 ]
Zhao, Tiejian [1 ]
Zhang, Zhiwei [2 ]
Liu, Xuemei [1 ]
Huang, Zhenqing [1 ]
Zhang, Yuan [1 ]
Li, Jingqiang [1 ]
机构
[1] Guangxi Univ Chinese Med, Dept Physiol, Nanning 530001, Guangxi, Peoples R China
[2] Guangxi Univ Chinese Med, Ruikang Hosp, Nanning 530021, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Plumbagin; Leptin; Hepatic stellate cell; Proliferation; Signal transduction pathway; Anti-inflammatory; TRANSFORMING-GROWTH-FACTOR; SIGNALING PATHWAYS; LEPTIN; ACTIVATION; EXPRESSION; GENE; INHIBITOR; APOPTOSIS; MIGRATION; JAK/STAT;
D O I
10.4314/tjpr.v12i5.5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Purpose: To investigate the protective effects of plumbagin against liver fibrosis and explore the influence of plumbagin on the proliferation of hepatic stellate cells (HSCs). Methods: HSC-LX2 cells were divided into blank/control group, 100 ng/ml leptin group, 100 ng/ml leptin + 2 mu mol/L plumbagin group, 100 ng/ml leptin + 8 mu mol/L plumbagin group and 100 ng/ml leptin + 6.25 mu g/ml colchicines group. The expressions of leptin receptor protein (OB-Rb), p-JAK2, p-STAT3, JAK2, STAT3, p-ERK1/2, ERK1/2 and MMP-1 were detected by Western blot assay. The content of type I collagen in the supernatant of HSCs was also measured after stimulation by leptin. Results: Leptin induced OB-Rb expression in HSCs, which reached a peak level at 24 h (p < 0.01). Leptin-induced OB-Rb expression was significantly inhibited by plumbagin at concentrations of 2 mu mol/L and 8 mu mol/L, respectively. Western blot assay revealed that plumbagin significantly decreased pJAK2 expression in leptin-treated HSCs (p < 0.01). Leptin induced expression of pSTAT3 significantly decreased after plumbagin treatment in HSC-LX2 (p < 0.01). p-ERK1/2 expression markedly decreased in plumbagin-treated HSCs (p < 0.01). Plumbagin significantly increased MMP-1 expression in leptin-treated HSCs (p < 0.01). Conclusion: Plumbagin has an anti-fibrotic effect and may decrease the protein expressions of components in JAK2-STAT3 pathway to inhibit HSC proliferation. Thus, plumbagin may be useful in the clinical prevention and treatment of liver fibrosis.
引用
收藏
页码:691 / 698
页数:8
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