Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue

被引：34

作者：

Babady, Ngolela Esther ^{[1
]}

Sloan, Lynne M. ^{[1
]}

Vetter, Emily A. ^{[1
]}

Patel, Robin ^{[1
,2
]}

Binnicker, Matthew J. ^{[1
]}

机构：

[1] Mayo Clin, Dept Lab Med & Pathol, Div Clin Microbiol, Rochester, MN 55905 USA

[2] Mayo Clin, Dept Med, Div Infect Dis, Rochester, MN 55905 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 62卷 / 04期

关键词：

Lyme; PCR; Blood; Cerebrospinal fluid; Synovial fluid;

D O I：

10.1016/j.diagmicrobio.2008.08.016

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We performed a retrospective review of 23,777 specimens tested by our Lyme real-time polymerase chain reaction assay to determine the percent positive rates by specimen source. The percent positive rates were highest in synovial fluid (6.4%) and tissue (6.5%), and lowest in blood (0.1%) and cerebrospinal fluid (0.09%). (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：464 / 466

页数：3

共 13 条

[1] Diagnosis of Lyme borreliosis [J].

Aguero-Rosenfeld, ME ;

Wang, GQ ;

Schwartz, I ;

Wormser, GP .

CLINICAL MICROBIOLOGY REVIEWS, 2005, 18 (03) :484-+

[2] Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis [J].

Brettschneider, S ;

Bruckbauer, H ;

Klugbauer, N ;

Hofmann, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2658-2665

[3] Evaluation of an internally controlled real-time PCR targeting the ospA gene for detection of Borrelia burgdorferi sensu lato DNA in cerebrospinal fluid [J].

Gooskens, J. ;

Templeton, K. E. ;

Claas, E. C. ;

van Dam, A. P. .

CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (09) :894-900

[4] BINDING OF HUMAN PLASMINOGEN TO BORRELIA-BURGDORFERI [J].

HU, LT ;

PERIDES, G ;

NORING, R ;

KLEMPNER, MS .

INFECTION AND IMMUNITY, 1995, 63 (09) :3491-3496

[5] Isolation, cloning, and expression of a 70-kilodalton plasminogen binding protein of Borrelia burgdorferi [J].

Hu, LT ;

Pratt, SD ;

Perides, G ;

Katz, L ;

Rogers, RA ;

Klempner, MS .

INFECTION AND IMMUNITY, 1997, 65 (12) :4989-4995

[6] DETECTION OF BORRELIA-BURGDORFERI BY NESTED POLYMERASE CHAIN-REACTION IN CEREBROSPINAL-FLUID AND URINE OF CHILDREN WITH NEUROBORRELIOSIS [J].

HUPPERTZ, HI ;

SCHMIDT, H ;

KARCH, H .

EUROPEAN JOURNAL OF PEDIATRICS, 1993, 152 (05) :414-417

[7] A LightCycler TaqMan assay for detection of Borrelia burgdorferi sensu lato in clinical samples [J].

Ivacic, Lynn ;

Reed, Kurt D. ;

Mitchell, Paul D. ;

Ghebranious, Nader .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 57 (02) :137-143

[8] DETECTION OF BORRELIA-BURGDORFERI DNA IN URINE SAMPLES AND CEREBROSPINAL-FLUID SAMPLES FROM PATIENTS WITH EARLY AND LATE LYME NEUROBORRELIOSIS BY POLYMERASE CHAIN-REACTION [J].

LEBECH, AM ;

HANSEN, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1646-1653

[9] A single-run, real-time PCR for detection and identification of Borrelia burgdorferi sensu lato species, based on the hbb gene sequence [J].

Portnoï, D ;

Sertour, N ;

Ferquel, E ;

Garnier, M ;

Baranton, G ;

Postic, D .

FEMS MICROBIOLOGY LETTERS, 2006, 259 (01) :35-40

[10] Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis [J].

Rauter, C ;

Mueller, M ;

Diterich, I ;

Zeller, S ;

Hassler, D ;

Meergans, T ;

Hartung, T .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (08) :910-917

← 1 2 →