The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice

被引：24

作者：

Anisimov, Andrey P. ^{[1
]}

Bakhteeva, Irina V. ^{[1
]}

Panfertsev, Evgeniy A. ^{[1
]}

Svetoch, Tat'yana E. ^{[1
]}

Kravchenko, Tat'yana B. ^{[1
]}

Platonov, Mikhail E. ^{[1
]}

Titareva, Galina M. ^{[1
]}

Kombarova, Tat'yana I. ^{[1
]}

Ivanov, Sergey A. ^{[1
]}

Rakin, Alexander V. ^{[2
]}

Amoako, Kingsley K. ^{[3
]}

Dentovskaya, Svetlana V. ^{[1
]}

机构：

[1] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Moscow Region, Russia

[2] Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany

[3] Canadian Food Inspect Agcy Lethbridge Lab, Lethbridge, AB T1J 3Z4, Canada

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2009年 / 58卷 / 01期

关键词：

PASTEURELLA PESTIS; CELLULAR PENETRATION; ESCHERICHIA-COLI; LIPOPOLYSACCHARIDE; PSEUDOTUBERCULOSIS; RESISTANCE; PROTEIN; CLONING; GENES; PSA;

D O I：

10.1099/jmm.0.005678-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and 1-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naive mice or animals immunized with this antigen.

引用

页码：26 / 36

页数：11

共 76 条

[41] Flea-borne transmission model to evaluate vaccine efficacy against naturally acquired bubonic plague [J].

Jarrett, CO ;

Sebbane, F ;

Adamovicz, JJ ;

Andrews, GP ;

Hinnebusch, BJ .

INFECTION AND IMMUNITY, 2004, 72 (04) :2052-2056

[42]

KARLYSHEV AV, 1994, NATO ASI H, V82, P321

[43] Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis [J].

Knirel, YA ;

Lindner, B ;

Vinogradov, EV ;

Kocharova, NA ;

Senchenkova, SN ;

Shaikhutdinova, RZ ;

Dentovskaya, SV ;

Fursova, NK ;

Bakhteeva, IV ;

Titareva, GM ;

Balakhonov, SV ;

Holst, O ;

Gremyakova, TA ;

Pier, GB ;

Anisimov, AP .

BIOCHEMISTRY, 2005, 44 (05) :1731-1743

[44]

Knirel YA, 2007, ADV EXP MED BIOL, V603, P88

[45]

KOVALEV OA, 1978, COR VASA, V20, P230

[46] Antibody profiling in plague patients by protein microarray [J].

Li, Bei ;

Zhou, Dongsheng ;

Wang, Zuyun ;

Song, Zhizhong ;

Wang, Hu ;

Li, Min ;

Dong, Xingqi ;

Wu, Mingshou ;

Guo, Zhaobiao ;

Yang, Ruifu .

MICROBES AND INFECTION, 2008, 10 (01) :45-51

[47] YERSINIA-PESTIS PH-6 ANTIGEN FORMS FIMBRIAE AND IS INDUCED BY INTRACELLULAR ASSOCIATION WITH MACROPHAGES [J].

LINDLER, LE ;

TALL, BD .

MOLECULAR MICROBIOLOGY, 1993, 8 (02) :311-324

[48] YERSINIA-PESTIS PH-6 ANTIGEN - GENETIC, BIOCHEMICAL, AND VIRULENCE CHARACTERIZATION OF A PROTEIN INVOLVED IN THE PATHOGENESIS OF BUBONIC PLAGUE [J].

LINDLER, LE ;

KLEMPNER, MS ;

STRALEY, SC .

INFECTION AND IMMUNITY, 1990, 58 (08) :2569-2577

[49] Effects of Psa and F1 on the adhesive and invasive interactions of Yersinia pestis with human respiratory tract epithelial cells [J].

Liu, Fengzhi ;

Chen, Huaiqing ;

Galvan, Estela M. ;

Lasaro, Melissa A. ;

Schifferli, Dieter M. .

INFECTION AND IMMUNITY, 2006, 74 (10) :5636-5644

[50] Transcriptional profiling of a mace plague model: insights into interaction between Yersinia pestis and its host [J].

Liu, Haihong ;

Wang, Hong ;

Qiu, Jingfu ;

Wang, Xiaoyi ;

Guo, Zhaobiao ;

Qiu, Yefeng ;

Zhou, Dongsheng ;

Han, Yanping ;

Du, Zongmin ;

Li, Cai ;

Song, Yajun ;

Yang, Ruifu .

JOURNAL OF BASIC MICROBIOLOGY, 2009, 49 (01) :92-99

← 1 2 3 4 5 6 7 8 →