The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice

被引：24

作者：

Anisimov, Andrey P. ^{[1
]}

Bakhteeva, Irina V. ^{[1
]}

Panfertsev, Evgeniy A. ^{[1
]}

Svetoch, Tat'yana E. ^{[1
]}

Kravchenko, Tat'yana B. ^{[1
]}

Platonov, Mikhail E. ^{[1
]}

Titareva, Galina M. ^{[1
]}

Kombarova, Tat'yana I. ^{[1
]}

Ivanov, Sergey A. ^{[1
]}

Rakin, Alexander V. ^{[2
]}

Amoako, Kingsley K. ^{[3
]}

Dentovskaya, Svetlana V. ^{[1
]}

机构：

[1] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Moscow Region, Russia

[2] Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany

[3] Canadian Food Inspect Agcy Lethbridge Lab, Lethbridge, AB T1J 3Z4, Canada

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2009年 / 58卷 / 01期

关键词：

PASTEURELLA PESTIS; CELLULAR PENETRATION; ESCHERICHIA-COLI; LIPOPOLYSACCHARIDE; PSEUDOTUBERCULOSIS; RESISTANCE; PROTEIN; CLONING; GENES; PSA;

D O I：

10.1099/jmm.0.005678-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and 1-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naive mice or animals immunized with this antigen.

引用

页码：26 / 36

页数：11

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