The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice

被引:24
作者
Anisimov, Andrey P. [1 ]
Bakhteeva, Irina V. [1 ]
Panfertsev, Evgeniy A. [1 ]
Svetoch, Tat'yana E. [1 ]
Kravchenko, Tat'yana B. [1 ]
Platonov, Mikhail E. [1 ]
Titareva, Galina M. [1 ]
Kombarova, Tat'yana I. [1 ]
Ivanov, Sergey A. [1 ]
Rakin, Alexander V. [2 ]
Amoako, Kingsley K. [3 ]
Dentovskaya, Svetlana V. [1 ]
机构
[1] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Moscow Region, Russia
[2] Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany
[3] Canadian Food Inspect Agcy Lethbridge Lab, Lethbridge, AB T1J 3Z4, Canada
关键词
PASTEURELLA PESTIS; CELLULAR PENETRATION; ESCHERICHIA-COLI; LIPOPOLYSACCHARIDE; PSEUDOTUBERCULOSIS; RESISTANCE; PROTEIN; CLONING; GENES; PSA;
D O I
10.1099/jmm.0.005678-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and 1-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naive mice or animals immunized with this antigen.
引用
收藏
页码:26 / 36
页数:11
相关论文
共 76 条
[1]  
Anisimov A. P., 2002, Molekulyarnaya Genetika Mikrobiologiya i Virusologiya, P3
[2]  
Anisimov A P, 2002, Mol Gen Mikrobiol Virusol, P3
[3]  
Anisimov AP, 2007, ADV EXP MED BIOL, V603, P23
[4]   Intraspecific diversity of Yersinia pestis [J].
Anisimov, AP ;
Lindler, LE ;
Pier, GB .
CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :434-+
[5]  
ANISIMOV AP, 1999, THESIS RUSSIAN RES A
[6]  
[Anonymous], 1989, Molecular Cloning: A Laboratory Manual
[7]  
Bakhteeva I.V., 2007, PROBL OSOBO OPASNYKH, V94, P40
[8]   NEWANTIGENIC COMPONENT OF PASTEURELLA PESTIS FORMED UNDER SPECIFIED CONDITIONS OF PH AND TEMPERATURE [J].
BENEFRAIM, S ;
BICHOWSKYSLOMNICKI, L ;
ARONSON, M .
JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :704-&
[9]   Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica [J].
Bengoechea, JA ;
Lindner, B ;
Seydel, U ;
Díaz, R ;
Moriyón, I .
MICROBIOLOGY-UK, 1998, 144 :1509-1515
[10]   BIOLOGICAL ACTIVITIES IN EXTRACTS OF PASTEURELLA PESTIS AND THEIR RELATION TO PH 6 ANTIGEN [J].
BICHOWSKYSLOMNICKI, L ;
BENEFRAIM, S .
JOURNAL OF BACTERIOLOGY, 1963, 86 (01) :101-+