Capillary Zone Electrophoresis Analysis for Kanamycins in Animal Tissue with Post-column Derivatization and Laser-induced Fluorescence

A selective and sensitive method for the determination of kanamycins with post-column derivatization in capillary zone electrophoresis and laser induced fluorescence detection was proposed. The derivatization reaction was carried out using a coaxial-gap reactor with a derivatization reagent of naphthalene-2, 3-dicarboxaldehyde( NDA ). The labeled kanamycins were detected with the excitation wavelength at 473 nm in laser induced fluorescence. The separation was carried out at - 15 kV with reversed electroosmotic flow in 50 mmol/L sodium acetic buffer ( pH 5. 0) containing 0. 5 mmol/L cetyltrimethyl ammonium. The derivatization was performed with the derivatization reagent of 1. 0 mmol/L thalene-2,3-dicarboxaldehyde, 8. 0 mmol/L 2-merc-aptoethanol and 35 mmol/L soldium ( pH 10. 0) in 30% ( V/V) methanol tetraborate buffer. The limits of detection for kanamycins ranging from 3. 6 x 10(-5) to 5. 2 x 10(-5) g/L and the separation efficiencies from 2.0 x 10(5) to 2. 4 x 10(5) theoretical plates were obtained. The method has been employed to analyze kanamycin residues in animal tissues.