Micromonospora equina sp nov., isolated from soil from a racecourse

被引:8
作者
Everest, Gareth J. [1 ]
Meyers, Paul R. [1 ]
机构
[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Cape Town, South Africa
基金
新加坡国家研究基金会; 英国医学研究理事会;
关键词
MAXIMUM-LIKELIHOOD; ROOT-NODULES; IDENTIFICATION; PHYLOGENY; SEQUENCES;
D O I
10.1099/ijs.0.042929-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two actinomycete strains were isolated from within the fynbos-rich area surrounded by the horseracing track at Kenilworth Racecourse in Cape Town, South Africa. Rapid molecular identification indicated that the isolates belonged to the family Micromonosporaceae. Based on 16S rRNA gene sequence BLAST analysis, the isolates were identified as members of the genus Micromonospora. Phylogenetic analysis showed that the isolates clustered with each other and were most closely related to Micromonospora viridifaciens DSM 43909(T). Further 16S rRNA gene sequence analysis using EzTaxon revealed that the isolates are closely related to Micromonospora auratinigra TT1-11(T), Micromonospora chaiyaphumensis MC5-1(T) Micromonospora eburnea LK2-10(T), Micromonospora nigra DSM 438181 and Micromonospora olivasterospora DSM 43868(T), DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of both isolates from related species; however, their high DNA-DNA relatedness showed that they belong to the same genomic species. Strain Y22(T) (=DSM 45644(T)=NRRL B-24859(T)) was selected as the type strain to represent this novel species, for which the name Micromonospora equina sp. nov. is proposed.
引用
收藏
页码:879 / 885
页数:7
相关论文
共 41 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]  
[Anonymous], 1989, Molecular Cloning: A Laboratory
[3]  
Atlas R.M., 2004, HDB MICROBIOLOGICAL
[4]   EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences [J].
Chun, Jongsik ;
Lee, Jae-Hak ;
Jung, Yoonyoung ;
Kim, Myungjin ;
Kim, Seil ;
Kim, Byung Kwon ;
Lim, Young-Woon .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007, 57 :2259-2261
[5]   Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns [J].
Cook, AE ;
Meyers, PR .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1907-1915
[6]  
Cross T., 1981, The prokaryotes. A handbook on habitats, isolation, and identification of bacteria. Volumes I and II, P2091
[7]  
Euzeby J. P., 2012, LIST OF PROKARYOTE N
[8]   Kribbella hippodromi sp nov., isolated from soil from a racecourse in South Africa [J].
Everest, Gareth J. ;
Meyers, Paul R. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008, 58 :443-446
[9]   The use of gyrB sequence analysis in the phylogeny of the genus Amycolatopsis [J].
Everest, Gareth J. ;
Meyers, Paul R. .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2009, 95 (01) :1-11
[10]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229