A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

被引：21
|
作者：
Liu, Z. B. [1 ]
Zhang, W. J. [1 ]
Gong, X. D. [3 ]
Zhang, Q. [4 ]
Zhou, L. R. [2 ,3 ]
机构：
[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresources & Ecoenvironm, Chengdu 610064, Peoples R China
[2] Sichuan Univ, Architecture & Environm Dept, Chengdu 610064, Sichuan, Peoples R China
[3] Univ British Columbia, Dept Civil Engn, Vancouver, BC V5Z 1M9, Canada
[4] Panzhihua Univ, Panzhihua, Sichuan, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Abiotic stress tolerance; Anti-oxidative enzymes; Cu/Zn-SOD; NaCl stress; Jatropha curcas; OXIDATIVE STRESS TOLERANCE; ANTIOXIDANT RESPONSES; ASCORBATE PEROXIDASE; DROUGHT-STRESS; NACL STRESS; PEA-PLANTS; EXPRESSION; GROWTH; OVEREXPRESSION; OXYGEN;
D O I：
10.4238/2015.March.20.19
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Superoxide dismutases (SODs) are involved in protecting plants against diverse biotic and abiotic stresses. In the present study, a novel Cu/Zn-SOD gene (JcCu/Zn-SOD) was cloned from Jatropha curcas L. Quantitative reverse transcription-polymerase chain reaction analysis revealed that JcCu/Zn-SOD is constitutively expressed in different tissues of J. curcas and induced under NaCl treatment. To characterize the function of this gene with respect to salt tolerance, the construct p35S:JcCu/Zn-SOD was developed and transformed into Arabidopsis using Agrobacterium-mediated transformation. Compared with wild-type, transgenic plants over-expressing JcCu/ Zn-SOD showed enhanced tolerance to salt stress during germination, seedling establishment, and growth in terms of longer root, larger rosette area, and a larger number of leaves in addition to higher SOD activity levels under NaCl stress. In addition, over-expression of JcCu/Zn-SOD resulted in lower monodialdehyde content in transgenic Arabidopsis compared to wild-type plants under the same NaCl stress. Therefore, JcCu/Zn-SOD can increase a plant salt stress tolerance potentially by reducing oxidant injury.
引用
收藏
页码：2086 / 2098
页数：13
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