Multilocus PCR Assays Elucidate Vegetative Incompatibility Gene Profiles of Cryphonectria parasitica in the United States

被引：34

作者：

Short, Dylan P. G. ^{[1
]}

Double, Mark ^{[1
]}

Nuss, Donald L. ^{[2
]}

Stauder, Cameron M. ^{[1
]}

MacDonald, William ^{[1
]}

Kasson, Matthew T. ^{[1
]}

机构：

[1] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA

[2] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2015年 / 81卷 / 17期

关键词：

CHESTNUT BLIGHT FUNGUS; COMPATIBILITY TYPES; BIOLOGICAL-CONTROL; POPULATION-STRUCTURE; VIRUS TRANSMISSION; AMERICAN CHESTNUTS; HIGH DIVERSITY; MATING-TYPE; HYPOVIRUS; RESISTANCE;

D O I：

10.1128/AEM.00926-15

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chestnut blight is a devastating disease of Castanea spp. Mycoviruses that reduce virulence (hypovirulence) of the causative agent, Cryphonectria parasitica, can be used to manage chestnut blight. However, vegetative incompatibility (vic) barriers that restrict anastomosis-mediated virus transmission hamper hypovirulence efficacy. In order to effectively determine the vegetative incompatibility genetic structure of C. parasitica field populations, we have designed PCR primer sets that selectively amplify and distinguish alleles for each of the six known diallelic C. parasitica vic genetic loci. PCR assay results were validated using a panel of 64 European tester strains with genetically determined vic genotypes. Analysis of 116 C. parasitica isolates collected from five locations in the eastern United States revealed 39 unique vic genotypes and generally good agreement between PCR and tester strain coculturing assays in terms of vic diversity and genotyping. However, incongruences were observed for isolates from multiple locations and suggested that the coculturing assay can overestimate diversity at the six known vic loci. The availability of molecular tools for rapid and precise vic genotyping significantly improves the ability to predict and evaluate the efficacy of hypovirulence and related management strategies.

引用

页码：5736 / 5742

页数：7

共 38 条

[1] CHESTNUT BLIGHT - THE CLASSICAL PROBLEM OF AN INTRODUCED PATHOGEN [J].

ANAGNOSTAKIS, SL .

MYCOLOGIA, 1987, 79 (01) :23-37

[2] POPULATION-DYNAMICS OF CRYPHONECTRIA-PARASITICA IN A MIXED-HARDWOOD FOREST IN CONNECTICUT [J].

ANAGNOSTAKIS, SL ;

KRANZ, J .

PHYTOPATHOLOGY, 1987, 77 (05) :751-754

[3] Programmed cell death correlates with virus transmission in a filamentous fungus [J].

Biella, S ;

Smith, ML ;

Aist, JR ;

Cortesi, P ;

Milgroom, MG .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2002, 269 (1506) :2269-2276

[4] Molecular Characterization of Vegetative Incompatibility Genes That Restrict Hypovirus Transmission in the Chestnut Blight Fungus Cryphonectria parasitica [J].

Choi, Gil H. ;

Dawe, Angus L. ;

Churbanov, Alexander ;

Smith, Myron L. ;

Milgroom, Michael G. ;

Nuss, Donald L. .

GENETICS, 2012, 190 (01) :113-U573

[5]

Cortesi P, 1998, APPL ENVIRON MICROB, V64, P2988

[6] Distribution and diversity of vegetative compatibility types in subpopulations of Cryphonectria parasitica in Italy [J].

Cortesi, P ;

Milgroom, MG ;

Bisiach, M .

MYCOLOGICAL RESEARCH, 1996, 100 :1087-1093

[7]

Cortesi P, 2001, GENETICS, V159, P107

[8] Fungi Associated with Hypovirulent Cankers of Differing Ages on American Chestnut [J].

Double, M. L. ;

Kolp, M. R. ;

Jarosz, A. M. ;

Baines, A. Davelos ;

Fulbright, D. W. ;

MacDonald, W. L. .

II EUROPEAN CONGRESS ON CHESTNUT, 2014, 1043 :57-65

[9] Evaluation of Cryphonectria parasitica Isolates Collected from the Great Smoky Mountains National Park [J].

Double, M. L. ;

MacDonald, W. L. ;

Taylor, G. M. .

V INTERNATIONAL CHESTNUT SYMPOSIUM, 2014, 1019 :85-89

[10]

Double ML, 1982, P USDA FOR SERV AM C, P145

← 1 2 3 4 →