Isolation and genetic identification of PAH degrading bacteria from a microbial consortium

被引：67

作者：

Carmen Molina, M. ^{[1
]}

Gonzalez, Natalia ^{[1
]}

Fernando Bautista, L. ^{[2
]}

Sanz, Raquel ^{[2
]}

Simarro, Raquel ^{[1
]}

Sanchez, Irene ^{[3
]}

Sanz, Jose L. ^{[3
]}

机构：

[1] Univ Rey Juan Carlos, Dept Biol & Geol, ESCET, Madrid 28933, Spain

[2] Univ Rey Juan Carlos, Dept Chem & Environm Technol, ESCET, Madrid 28933, Spain

[3] Univ Autonoma Madrid, Dept Mol Biol, E-28049 Madrid, Spain

来源：

BIODEGRADATION | 2009年 / 20卷 / 06期

关键词：

Biodegradation; Polycyclic aromatic hydrocarbons; Toxicity; Soil; Pseudomonas; Enterobacter; Stenotrophomonas; POLYCYCLIC AROMATIC-HYDROCARBONS; CRUDE-OIL; BIODEGRADATION; BIOREMEDIATION; PSEUDOMONAS; CONFIDENCE; PARAMETERS; SEDIMENTS;

D O I：

10.1007/s10532-009-9267-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polycyclic aromatic hydrocarbons (PAH; naphthalene, anthracene and phenanthrene) degrading microbial consortium C2PL05 was obtained from a sandy soil chronically exposed to petroleum products, collected from a petrochemical complex in Puertollano (Ciudad Real, Spain). The consortium C2PL05 was highly efficient degrading completely naphthalene, phenanthrene and anthracene in around 18 days of cultivation. The toxicity (Microtox (TM) method) generated by the PAH and by the intermediate metabolites was reduced to levels close to non-toxic in almost 40 days of cultivation. The identified bacteria from the contaminated soil belonged to gamma-proteobacteria and could be include in Enterobacter and Pseudomonas genus. DGGE analysis revealed uncultured Stenotrophomonas ribotypes as a possible PAH degrader in the microbial consortium. The present work shows the potential use of these microorganisms and the total consortium for the bioremediation of PAH polluted areas since the biodegradation of these chemicals takes place along with a significant decrease in toxicity.

引用

页码：789 / 800

页数：12

共 45 条

[1]

Aitken MD, 2004, SOIL BIOL, V2, P83

[2] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[3] Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence [J].

Anzai, Y ;

Kim, H ;

Park, JY ;

Wakabayashi, H ;

Oyaizu, H .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1563-1589

[4]

BOSSERT I, 1984, APPL ENVIRON MICROB, V47, P763, DOI 10.1128/AEM.47.4.763-767.1984

[5] Uptake and active efflux of polycyclic aromatic hydrocarbons by Pseudomonas fluorescens LP6a [J].

Bugg, T ;

Foght, JM ;

Pickard, MA ;

Gray, MR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5387-5392

[6] Effects of nutrient concentration on the biodegradation of crude oil and associated microbial populations in the soil [J].

Chaîneau, CH ;

Rougeux, G ;

Yéprémian, C ;

Oudot, J .

SOIL BIOLOGY & BIOCHEMISTRY, 2005, 37 (08) :1490-1497

[7] Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge [J].

Cui, Zhisong ;

Lai, Qiliang ;

Dong, Chunming ;

Shao, Zongze .

ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (08) :2138-2149

[8] Effectiveness of bioremediation of crude oil contaminated subantarctic intertidal sediment: The microbial response [J].

Delille, D ;

Delille, B ;

Pelletier, E .

MICROBIAL ECOLOGY, 2002, 44 (02) :118-126

[9] EFFECT OF ENVIRONMENTAL PARAMETERS ON THE BIODEGRADATION OF OIL SLUDGE [J].

DIBBLE, JT ;

BARTHA, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1979, 37 (04) :729-739

[10] Measurement of biodegradability parameters for single unsubstituted and methylated polycyclic aromatic hydrocarbons in liquid bacterial suspensions [J].

Dimitriou-Christidis, Petros ;

Autenrieth, Robin L. ;

McDonald, Thomas J. ;

Desai, Anuradha M. .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 97 (04) :922-932

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