MiR-4782-3p suppresses glioma migration and invasion by regulating p-cadherin

MicroRNAs (miRNAs), a class of non-coding endogenous RNAs, were found to be involved in the regulation of tumor progression with post-transcriptional function. The aim of present study was to explore the role of miR-4782-3p in the development of glioma migration and invasion. Our findings indicated that the expression of miR-4782-3p was significantly repressed in glioma tissues compared to the adjacent normal tissues. In addition, our results demonstrated that miR-4782-3p could suppress the migration and invasion ability of U251 and U87 glioma cells. We also validated P-Cadherin (CDH3) as the potential target gene of miR-4782-3p using an enhanced green fluorescence protein (EGFP) reporter assay. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were employed and confirmed that miR-4782-3p suppressed CDH3 mRNA and protein levels. The results suggested that miR-4782-3p could negatively regulate the expression of CDH3 by directly binding to the 3'-UTR of CDH3. We further found that CDH3-dependent cell mobility was associated with p120 catenin (p120ctn) transition from the membrane to the cytoplasm. In conclusion, our findings indicated that a miR-47823p/CDH3/p120ctn pathway was strongly related to the regulation of glioma cell migration and invasion.