A Pilot Study for Metabolic Profiling of Obesity-Associated Microbial Gut Dysbiosis in Male Wistar Rats

被引:4
作者
Hernandez-Baixauli, Julia [1 ]
Puigbo, Pere [1 ,2 ,3 ]
Torrell, Helena [4 ]
Palacios-Jordan, Hector [4 ]
Ripoll, Vicent J. Ribas [5 ]
Caimari, Antoni [1 ]
Del Bas, Josep M. [1 ]
Baselga-Escudero, Laura [1 ]
Mulero, Miquel [6 ]
机构
[1] Eurecat, Ctr Tecnol Catalunya, Unitat Nutr & Salut, Reus 43204, Spain
[2] Univ Rovira & Virgili, Dept Biochem & Biotechnol, Tarragona 43007, Spain
[3] Univ Turku, Dept Biol, Turku 20014, Finland
[4] Eurecat, Joint Unit Univ Rovira & Virgili EURECAT, Ctr Tecnol Catalunya, Ctr Omic Sci COS, Reus 43204, Spain
[5] Eurecat, Ctr Tecnol Catalunya, EHlth Unit, Barcelona 08005, Spain
[6] Univ Rovira & Virgili, Nutrigen Res Grp, Dept Biochem & Biotechnol, Tarragona 43007, Spain
关键词
microbial dysbiosis; gut microbiota; metagenomics; metabolomics; dysbiosis biomarkers; metabolic profile; diacylglycerol 34:2; hippurate; 3-HPPA; o-coumaric acid; HIGH-FAT DIET; HIPPURIC-ACID; INTESTINAL MICROBIOTA; INSULIN-RESISTANCE; DIABETES-MELLITUS; DIACYLGLYCEROL; DISEASE; LIVER; BIOMARKERS; MECHANISM;
D O I
10.3390/biom11020303
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obesity is one of the most incident and concerning disease worldwide. Definite strategies to prevent obesity and related complications remain elusive. Among the risk factors of the onset of obesity, gut microbiota might play an important role in the pathogenesis of the disease, and it has received extensive attention because it affects the host metabolism. In this study, we aimed to define a metabolic profile of the segregated obesity-associated gut dysbiosis risk factor. The study of the metabolome, in an obesity-associated gut dysbiosis model, provides a relevant way for the discrimination on the different biomarkers in the obesity onset. Thus, we developed a model of this obesity risk factors through the transference of gut microbiota from obese to non-obese male Wistar rats and performed a subsequent metabolic analysis in the receptor rats. Our results showed alterations in the lipid metabolism in plasma and in the phenylalanine metabolism in urine. In consequence, we have identified metabolic changes characterized by: (1) an increase in DG:34:2 in plasma, a decrease in hippurate, (2) an increase in 3-HPPA, and (3) an increase in o-coumaric acid. Hereby, we propose these metabolites as a metabolic profile associated to a segregated dysbiosis state related to obesity disease.
引用
收藏
页码:1 / 22
页数:22
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