Recombinant chromosomes of advanced backcross plants between Allium cepa L. and A-fistulosum L. revealed by in situ hybridization

被引：7
作者：
Hou, A
Peffley, EB [1 ]
机构：
[1] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA
[2] Texas Tech Univ, Inst Biotechnol, Lubbock, TX 79409 USA
来源：
THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 08期
关键词：
fluorescence in situ hybridization; genomic in situ hybridization; interspecific introgression; Japanese bunching onion; onion;
D O I：
10.1007/s001220051423
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Cytological analysis of (Allium cepa L.xAllium fistulosum L.)xA. cepa L. F1BC3 plants revealed most plants were diploid with 16 chromosomes. Karyotypes of these plants showed recombinant chromosomes. Fluorescence and genomic in situ hybridization patterns of interspecific F-1 hybrid and F1BC3 plants revealed A. fistulosum chromosomes or chromosomal segments. A highly repetitive 376-bp DNA sequence and genomic DNA of A. fistulosum revealed similar telomeric hybridization sites when hybridized onto A. fistulosum chromosomes. Cytogenetic evidence showed that A. fistulosum DNA has recombined into the A. cepa genome.
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页码：1190 / 1196
页数：7
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