Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

被引：348

作者：

Watanabe, Satoshi

Hideshima, Rumiko ^{[3
]}

Xia, Zhengjun

Tsubokura, Yasutaka

Sato, Shusei ^{[2
]}

Nakamoto, Yumi ^{[4
]}

Yamanaka, Naoki ^{[5
]}

Takahashi, Ryoji ^{[6
]}

Ishimoto, Masao ^{[4
]}

Anai, Toyoaki ^{[3
]}

Tabata, Satoshi ^{[2
]}

Harada, Kyuya ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Soybean Genome Res Team, Tsukuba, Ibaraki 3058602, Japan

[2] Kazusa DNA Res Inst, Chiba 2920812, Japan

[3] Saga Univ, Fac Agr, Saga 8408502, Japan

[4] Natl Agr Res Ctr Hokkaido Reg, Sapporo, Hokkaido 0628555, Japan

[5] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan

[6] Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan

来源：

GENETICS | 2009年 / 182卷 / 04期

关键词：

PHYTOCHROME-A; FLOWERING TIME; AGRONOMIC TRAITS; GENOMIC ANALYSIS; FUSARIUM-SOLANI; QTL ANALYSIS; LINKAGE MAP; RESISTANCE; GLYCINE; DUPLICATION;

D O I：

10.1534/genetics.108.098772

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Photosensitivity plays an essential role in the response of plants to their changing environments throughout life cycle. In soybean [Glycine max (L.) Merrill], several associations between photosensitivity and maturity loci are known, but only limited information at the molecular level is available. The FT3 locus is one of the quantitative trait loci (QTL) for flowering time that corresponds to the maturity locus E3. To identify the gene responsible for this QTL, a map-based cloning strategy was undertaken. One phytochrome A gene (GmPhyA3) was considered a strong candidate for the FT3 locus. Allelism tests and gene sequence comparisions showed that alleles of Misuzudaizu (FT3/FT3; JP28856) and L62-667 (e3/e3; PI547716) showed weak or complete loss of function, respectively. High red/far-red (R/FR) long-day conditions enhanced the effects of the E3/FT3 alleles in various genetic backgrounds. Moreover, a mutant line harboring the nonfunctional GmPhyA3 flowered earlier than the original Bay (E3/E3; PI553043) under similar conditions. These results suggest that the variation in phytochrome A may contribute to the complex systems of soybean flowering response and geogrphic adaptation.

引用

页码：1251 / 1262

页数：12

共 66 条

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