Exogenous, but not endogenous, nitric oxide increases proliferation rates in senescent human fibroblasts

被引：42

作者：

Gansauge, S ^{[1
]}

Gansauge, F ^{[1
]}

Nussler, AK ^{[1
]}

Rau, B ^{[1
]}

Poch, B ^{[1
]}

Schoenberg, MH ^{[1
]}

Beger, HG ^{[1
]}

机构：

[1] UNIV ULM, DEPT GEN SURG, DIV MOL ONCOL, D-89075 ULM, GERMANY

来源：

FEBS LETTERS | 1997年 / 410卷 / 2-3期

关键词：

fibroblast; human; nitric oxide; proliferation;

D O I：

10.1016/S0014-5793(97)00544-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the effects of endogenously produced and exogenously applied nitric oxide (NO) on cell proliferation rates and cell cycle regulation in senescent human fibroblasts (WI38), Induction of inducible nitric oxide synthase by tumor necrosis factor-alpha, interferon-gamma and interleukin-1 beta inhibited cell proliferation and led to a G1 arrest. These effects were partially reversible by N-G-monomethyl-arginine (NMA). Addition of the NO donors sodium nitroprusside (SNP) or S-nitroso-N-acetylpenicillamine (SNAP) increased cell proliferation rates as well as the S/G2 fraction, This points to a functional role of NO in cell cycle regulation and cell proliferation in human fibroblasts which depends on the mode of NO generation as well as the culture conditions used. (C) 1997 Federation of European Biochemical Societies.

引用

页码：160 / 164

页数：5

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