Aptamer-Directed Conjugation of DNA to Therapeutic Antibodies

被引：14

作者：

Skovsgaard, Mikkel B. ^{[1
]}

Mortensen, Michael R. ^{[1
]}

Palmfeldt, Johan ^{[2
]}

Gothelf, Kurt, V ^{[1
]}

机构：

[1] Aarhus Univ, Dept Chem, iNANO, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Dept Clin Med, Palle Juul Jensens Blvd 99, DK-8200 Aarhus N, Denmark

来源：

BIOCONJUGATE CHEMISTRY | 2019年 / .30卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

TRANSFERRIN; PROTEINS; RECEPTOR; RNA;

D O I：

10.1021/acs.bioconjchem.9b00363

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for aptamer directed conjugation of DNA to therapeutic antibodies has been developed. In the method, an antibody selective aptamer binds to a specific site in the constant domain of human IgG1 antibodies and is used for both templated and direct conjugation to the antibodies. Through optimization of the design and reaction conditions, the antibody-DNA conjugates could be produced efficiently using equal stoichiometry of protein and DNA. Three different antibodies were evaluated, and the conjugates were characterized by anion exchange chromatography and SDS-PAGE. The conjugation sites for one of the antibodies were determined by MS/MS analysis of the digested conjugate. The antibody-DNA conjugate was also tested for receptor binding on cell surfaces showing retained binding.

引用

页码：2127 / 2135

页数：9

共 50 条

[1] Protein nanoarrays - Aptamer-directed self-assembly of protein arrays on a DNA nanostructure
Liu, Y
Lin, CX
Li, HY
Yan, H
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (28) : 4333 - 4338
[2] Novel strategy for aptamer-directed nanovesicle targeting in cholangiocarcinoma
Yu, Mincheng
Tomlinson, Jennifer L.
Loeuillard, Emilien J.
Watkins, Ryan D.
Conboy, Caitlin B.
Takaichi, Shohei
Werneburg, Nathan W.
Alva-Ruiz, Roberto
Abdelrahman, Amro
Carlson, Danielle M.
Gores, Gregory J.
Yang, Jingchun
Ilyas, Sumera I.
Patel, Tushar
Smoot, Rory L.
CANCER RESEARCH, 2023, 83 (07)
[3] Multi-functional DNA-conjugated nanohydrogels for aptamer-directed breast cancer cell targeting
Jiang, Gangfeng
Wang, Nannan
Jia, Lina
Che, Hongli
Wang, Lihui
Yang, Jingyu
Xu, Hui
Wu, Chunfu
Liu, Mingzhe
NEW JOURNAL OF CHEMISTRY, 2021, 45 (43) : 20410 - 20418
[4] Double-Network DNA Macroporous Hydrogel Enables Aptamer-Directed Cell Recruitment to Accelerate Bone Healing
Miao, Yali
Liu, Xiao
Luo, Jinshui
Yang, Qian
Chen, Yunhua
Wang, Yingjun
ADVANCED SCIENCE, 2024, 11 (01)
[5] Aptamer-directed lanthanide chelate self-assembly for rapid thrombin detection
Pakkila, Henna
Blom, Sami
Kopra, Kari
Soukka, Tero
ANALYST, 2013, 138 (17) : 5107 - 5112
[6] A Difunctional Regeneration Scaffold for Knee Repair based on Aptamer-Directed Cell Recruitment
Hu, Xiaoxia
Wang, Yulan
Tan, Yaning
Wang, Jie
Liu, Haoyang
Wang, Yingqian
Yang, Shuang
Shi, Miusi
Zhao, Shiyong
Zhang, Yufeng
Yuan, Quan
ADVANCED MATERIALS, 2017, 29 (15)
[7] Aptamer-Directed Bidirectional Modulation of Vascular Niches for Promoted Regeneration of Segmental Trachea Defect
Cao, Runfeng
Chen, Zhenying
Ye, Qing
Sun, Weiyan
Lin, Weikang
Tang, Hai
Yang, Xingseng
Liang, Junhao
Chen, Yi
Wang, Lei
Bai, Qingfeng
Pan, Ziying
Hu, Yulong
Xie, Dong
Zhao, Deping
Hu, Yong
Chen, Chang
ADVANCED FUNCTIONAL MATERIALS, 2025, 35 (12)
[8] Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells
Chen, Xigao
Qiu, Liping
Cai, Ren
Cui, Cheng
Li, Long
Jiang, Jian-hui
Tan, Weihong
ACS APPLIED MATERIALS & INTERFACES, 2020, 12 (34) : 37845 - 37850
[9] Aptamer-Directed Synthesis of Multifunctional Lanthanide-Doped Porous Nanoprobes for Targeted Imaging and Drug Delivery
Zhou, Li
Li, Zhenhua
Ju, Enguo
Liu, Zhen
Ren, Jinsong
Qu, Xiaogang
SMALL, 2013, 9 (24) : 4262 - 4268
[10] Engineering aptamer-directed phosphatase recruiting chimeras: a strategy for modulating receptor function and overcoming drug resistance
Zhilan Zhou
Yichang Liu
Ya Wang
Hang Jiang
Tingting Chen
Yingdi Zhu
Ting Fu
Juan Li
Nature Communications, 16 (1)

← 1 2 3 4 5 →