A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans

被引：14

作者：

Reilly, Morgann C. ^{[1
]}

Levery, Steven B. ^{[2
]}

Castle, Sherry A. ^{[3
]}

Klutts, J. Stacey ^{[1
,4
]}

Doering, Tamara L. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[2] Univ Copenhagen, Dept Cellular & Mol Med, DK-2200 Copenhagen N, Denmark

[3] Univ New Hampshire, Dept Chem, Durham, NH 03824 USA

[4] Univ Iowa, Dept Pathol, Carver Coll Med & Pathol & Lab Med, Iowa City, IA 52246 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 52期

基金：

美国国家卫生研究院;

关键词：

STRUCTURE MUTANT STRAINS; CAPSULE-ASSOCIATED GENE; GLUCURONIC ACID; VIRULENCE; GLYCOSYLTRANSFERASES; XYLOSE; BETA-1,2-XYLOSYLTRANSFERASE; GALACTOXYLOMANNAN; IDENTIFICATION; MECHANISMS;

D O I：

10.1074/jbc.M109.056226

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.

引用

页码：36118 / 36127

页数：10

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