Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm

被引：21

作者：

Xu, Dongdong ^{[1
,2
]}

Yoshino, Tasuku ^{[3
]}

Konishi, Junpei ^{[3
]}

Yoshikawa, Hiroyuki ^{[4
]}

Ino, Yasuko ^{[4
]}

Yazawa, Ryosuke ^{[3
]}

dos Santos Nassif Lacerda, Samyra Maria ^{[5
]}

de Franca, Luiz Renato ^{[5
]}

Takeuchi, Yutaka ^{[2
,6
]}

机构：

[1] Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan, Zhejiang, Peoples R China

[2] Kagoshima Univ, Fac Fisheries, Div Fisheries Resource Sci, Shimoarata 4-50-20, Kagoshima 8900056, Japan

[3] Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Minato Ku, 4-5-7 Konan, Tokyo, Japan

[4] Natl Fisheries Univ, Japan Fisheries Res & Educ Agcy, Dept Appl Aquabiol, 2-7-1 Nagata Honmachi, Shimonoseki, Yamaguchi, Japan

[5] Univ Fed Minas Gerais, Dept Morphol, Lab Cellular Biol, Belo Horizonte, MG, Brazil

[6] Kagoshima Univ, United Grad Sch Agr Sci, Kagoshima, Japan

来源：

BIOLOGY OF REPRODUCTION | 2019年 / 101卷 / 02期

基金：

中国国家自然科学基金; 日本学术振兴会; 奥地利科学基金会;

关键词：

surrogate broodstock; fish reproduction; gametogenesis; spermatogenesis; spermatogonia; testis; transplantation; male infertility; REPRODUCTIVELY STERILE FISH; SEXUAL-DIMORPHISM; LINE; SPERMATOGENESIS; ZEBRAFISH; TELEOST; GENERATION; PROGRESS;

D O I：

10.1093/biolre/ioz045

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An interspecific hybrid marine fish that developed a testis-like gonad without any germ cells, i.e., a germ cell-less gonad, was produced by hybridizing a female blue drum Nibea mitsukurii with a male white croaker Pennahia argentata. In this study, we evaluated the suitability of the germ cell-less fish as a recipient by transplanting donor testicular cells directly into the gonads through the urogenital papilla. The donor testicular cells were collected from hemizygous transgenic, green fluorescent protein (gfp) (+/-) blue drum, and transplanted into the germ cell-less gonads of the 6-month-old adult hybrid croakers. Fluorescent and histological observations showed the colonization, proliferation, and differentiation of transplanted spermatogonial cells in the gonads of hybrid croakers. The earliest production of spermatozoa in a hybrid recipient was observed at 7 weeks post-transplantation (pt), and 10% of the transplanted recipients produced donor-derived gfp-positive spermatozoa by 25 weeks pt. Sperm from the hybrid recipients were used to fertilize eggs from wild-type blue drums, and approximately 50% of the resulting offspring were gfp-positive, suggesting that all offspring originated from donor-derived sperm that were produced in the transplanted gfp (+/-) germ cells. To the best of our knowledge, this is the first report of successful spermatogonial transplantation using a germ cell-less adult fish as a recipient. This transplantation system has considerable advantages, such as the use of comparatively simple equipment and procedures, and rapid generation of donor-derived spermatogenesis and offspring, and presents numerous applications in commercial aquaculture. Summary Sentence Hybrid marine sciaenid fish showing germ cell-less phenotype are ideal recipients for developing spermatogonial transplantation in adult fish, and enable the rapid production of donor-derived sperm in 7 weeks post-transplantation.

引用

页码：492 / 500

页数：9

共 43 条

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