Immunochromatographic rK39 strip test in the serodiagnosis of visceral leishmaniasis in Tunisia

被引：12
|
作者：
Saghrouni, F. [2 ]
Gaied-Meksi, S. [2 ]
Fathallah, A. [1 ,2 ]
Amri, F. [3 ]
Ach, H. [2 ]
Guizani, I. [1 ]
Ben Said, M. [2 ]
机构：
[1] Inst Pasteur, Lab Epidemiol & Ecol Parasitaire, Res Programme Appl Genom & Biotechnol Parasit Dis, Tunis, Tunisia
[2] Hop Farhat Hached, Parasitol Lab, Sousse, Tunisia
[3] Hop Ibn El Jazzar, Serv Pediat, Kairouan, Tunisia
来源：
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 103卷 / 12期
关键词：
Visceral leishmaniasis; Diagnosis; rK39 strip test; Immunofluorescent; antibody test; Sensitivity and specificity; Tunisia; DIRECT AGGLUTINATION-TEST; LINKED-IMMUNOSORBENT-ASSAY; RECOMBINANT K-39 ANTIGEN; DONOVANI INFECTION; DIPSTICK TEST; KALA-AZAR; CUTANEOUS LEISHMANIASIS; DIAGNOSIS; ANTIBODY; CHAGASI;
D O I：
10.1016/j.trstmh.2008.10.034
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The performance of the rK39 strip test in the diagnosis of Tunisian visceral leishmaniasis (VL) was evaluated and compared with that of immunofluorescent antibody test (IFAT). A total of 929 sera, including 574 from VL patients, 54 from cutaneous leishmaniasis (CL) patients, 42 from patients with other protozoan diseases, 152 from patients with non-parasitic diseases and 107 from healthy controls, were used in the study. The sensitivity and specificity of the rK39 strip test were 87.1 and 94.4%, respectively. Sixteen CL sera showed positive results, suggesting that the rK39 strip test is not restricted to Leishmania donovani complex detection. IFAT was comparatively more sensitive (98.9%) but slightly less specific (90.7%). Despite cross-reactivity shown by CL sera, the rK39 strip test can be recommended for the routine diagnosis of VL in Tunisia, as VL and CL are distinct clinical. entities. (C) 2008 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：1273 / 1278
页数：6
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