Serine 62 is a phosphorylation site in folliculin, the Birt-Hogg-Dube gene product

被引：18

作者：

Wang, Lu

Kobayashi, Toshiyuki

Piao, Xianghua

Shiono, Masatoshi

Takagi, Yumiko

Mineki, Reiko ^{[2
]}

Taka, Hikari ^{[2
]}

Zhang, Danqing

Abe, Masaaki

Sun, Guodong

Hagiwara, Yoshiaki ^{[3
]}

Okimoto, Kazuo ^{[4
]}

Matsumoto, Izumi ^{[5
]}

Kouchi, Mami ^{[5
]}

Hino, Okio ^{[1
]}

机构：

[1] Juntendo Univ, Sch Med, Dept Pathol & Oncol, Bunkyo Ku, Tokyo 1138421, Japan

[2] Juntendo Univ, Sch Med, Div Prote & Biomol Sci, Tokyo 1138421, Japan

[3] Immunobiol Labs Co Ltd, Res & Dev, Fujioka, Gunma 3750005, Japan

[4] Dainippon Sumitomo Pharma Co Ltd, Res Adm, Osaka 5640053, Japan

[5] Dainippon Sumitomo Pharma Co Ltd, Safety Res Labs, Osaka 5540022, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 01期

关键词：

Birt-Hogg-Dube syndrome; Folliculin; 5 '-AMP-activated protein kinase; Phosphorylation; FLCN-interacting protein; BHD GENE; PROTEIN; IDENTIFICATION;

D O I：

10.1016/j.febslet.2009.11.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, it was reported that the product of Birt-Hogg-Dube syndrome gene (folliculin, FLCN) is directly phosphorylated by 5'-AMP-activated protein kinase (AMPK). In this study, we identified serine 62 (Ser62) as a phosphorylation site in FLCN and generated an anti-phospho-Ser62-FLCN antibody. Our analysis suggests that Ser62 phosphorylation is indirectly up-regulated by AMPK and that another residue is directly phosphorylated by AMPK. By binding with FLCN-interacting proteins (FNIP1 and FNIP2/FNIPL), Ser62 phosphorylation is increased. A phospho-mimic mutation at Ser62 enhanced the formation of the FLCN-AMPK complex. These results suggest that function(s) of FLCN-AMPK-FNIP complex is regulated by Ser62 phosphorylation.

引用

页码：39 / 43

页数：5

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