ANGIOGENIC POTENTIAL OF HUMAN DENTAL PULP STROMAL (STEM) CELLS

被引:84
作者
Marchionni, C. [1 ]
Bonsi, L. [1 ,6 ]
Alviano, F. [1 ]
Lanzoni, G. [1 ]
Di Tullio, A. [1 ]
Costa, R. [1 ]
Montanari, M. [2 ]
Tazzari, P. L. [3 ]
Ricci, F. [3 ]
Pasquinelli, G. [4 ]
Orrico, C. [5 ]
Grossi, A. [1 ]
Prati, C. [2 ]
Bagnara, G. P. [1 ,6 ]
机构
[1] Univ Bologna, Dept Histol Embryol & Appl Biol, I-40126 Bologna, Italy
[2] Univ Bologna, Dept Oral Sci, Endodont Unit, I-40126 Bologna, Italy
[3] St Orsola Marcello Malpighi Hosp, Transfus Med Serv, Bologna, Italy
[4] St Orsola Marcello Malpighi Hosp, Dept Radiol & Histocytopathol Clin Sci, Bologna, Italy
[5] Univ Bologna, Dept Specialist Surg & Anaesthesiol Sci, Bologna, Italy
[6] Univ Bologna, Giorgio Prodi Interdept Ctr Canc Res, Bologna, Italy
关键词
dental pulp; stem cells; VEGF; angiogenesis; GROWTH-FACTORS; EXPRESSION; INDUCTION; COLLAGEN; FLT-1;
D O I
10.1177/039463200902200315
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dental pulp is a heterogeneous microenviroment where unipotent progenitor and pluripotent mesenchymal stem cells cohabit. In this study we investigated whether human Dental Pulp Stromal (Stem) Cells (DP-SCs) committed to the angiogenic fate. DP-SCs showed the specific mesenchymal immunophenotypical profile positive for CD29, CD44, CD73, CD105, CD166 and negative for CD14, CD34, CD45, in accordance with that reported for bone marrow-derived SCs. The Oct-4 expression in DP-SCs, evaluated through RT-PCR analysis, increased in relation with the number of the passages in cell culture and decreased after angiogenic induction. In agreement with their multipotency, DP-SCs differentiated toward osteogenic and adipogenic commitments. In angiogenic experiments, differentiation of DP-SCs, through Vascular Endothelial Growth Factor (VEGF) induction, was evaluated by in vitro matrigel assay and by cytometric analysis. Accordingly, endothelial-specific markers like Flt-1 and KDR were basally expressed and they increased after exposure to VEGF together with the occurrence of ICAM-1 and von Willebrand Factor positive cells. In addition, VEGF-induced DP-SCs maintained endothelial cell-like features when cultured in a 3-D fibrin mesh, displaying focal organization into capillary-like structures. The DP-SC angiogenic potential may prove a remarkable tool for novel approaches to developing tissue-engineered vascular grafts which are useful when vascularization of ischemic tissues is required.
引用
收藏
页码:699 / 706
页数:8
相关论文
共 28 条
[1]   Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures [J].
Alliot-Licht, B ;
Bluteau, G ;
Magne, D ;
Lopez-Cazaux, S ;
Lieubeau, B ;
Daculsi, G ;
Guicheux, J .
CELL AND TISSUE RESEARCH, 2005, 321 (03) :391-400
[2]   Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro [J].
Alviano, Francesco ;
Fossati, Valentina ;
Marchionni, Cosetta ;
Arpinati, Mario ;
Bonsi, Laura ;
Franchina, Michele ;
Lanzoni, Giacomo ;
Cantoni, Silvia ;
Cavallini, Claudia ;
Bianchi, Francesca ;
Tazzari, Pier Luigi ;
Pasquinelli, Gianandrea ;
Foroni, Laura ;
Ventura, Carlo ;
Grossi, Alberto ;
Bagnara, Gian Paolo .
BMC DEVELOPMENTAL BIOLOGY, 2007, 7
[3]   In vitro stem cell cultures from human dental pulp and periodontal ligament:: New prospects in dentistry [J].
Ballini, A. ;
De Frenza, G. ;
Cantore, S. ;
Papa, F. ;
Grano, M. ;
Mastrangelo, F. ;
Tete, S. ;
Grassi, F. R. .
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2007, 20 (01) :9-16
[4]  
BERTOLD PM, 1998, BIOL PERIODONTAL CON, P241
[5]   Injectable fibrin scaffold improves cell transplant survival, reduces infarct expansion, and induces neovasculature formation in ischemic myocardium [J].
Christman, KL ;
Vardanian, AJ ;
Fang, QZ ;
Sievers, RE ;
Fok, HH ;
Lee, RJ .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2004, 44 (03) :654-660
[6]   Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation [J].
d'Aquino, R. ;
Graziano, A. ;
Sampaolesi, M. ;
Laino, G. ;
Pirozzi, G. ;
De Rosa, A. ;
Papaccio, G. .
CELL DEATH AND DIFFERENTIATION, 2007, 14 (06) :1162-1171
[7]  
FINKELMAN RD, 1990, J BONE MINER RES, V5, P717
[8]   Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction [J].
Gandia, Carolina ;
Arminan, Ana ;
Garcia-Verdugo, Jose Manuel ;
Lledo, Elisa ;
Ruiz, Amparo ;
Minana, M. Dolores ;
Sanchez-Torrijos, Jorge ;
Paya, Rafael ;
Mirabet, Vicente ;
Carbonell-Uberos, Francisco ;
Llop, Mauro ;
Montero, Jose Anastasio ;
Sepulveda, Pilar .
STEM CELLS, 2008, 26 (03) :638-645
[9]  
Gronthos S, 2004, ADULT STEM CELLS, P67
[10]  
Looijenga LHJ, 2003, CANCER RES, V63, P2244