Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994-2010

被引：33

作者：

Bok, M. ^{[1
]}

Mino, S. ^{[1
,2
]}

Rodriguez, D. ^{[1
]}

Badaracco, A. ^{[1
]}

Nunes, I. ^{[3
]}

Souza, S. P. ^{[3
]}

Bilbao, G. ^{[4
]}

Louge Uriarte, E. ^{[5
]}

Galarza, R. ^{[6
]}

Vega, C. ^{[1
]}

Odeon, A. ^{[4
]}

Saif, L. J. ^{[7
]}

Parreno, V. ^{[1
]}

机构：

[1] Natl Inst Agr Technol INTA, CICVyA, Inst Virol, Buenos Aires, DF, Argentina

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China

[3] Univ Sao Paulo, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, BR-05508 Sao Paulo, Brazil

[4] EEA INTA Balcarce, Hlth Anim Lab, Buenos Aires, DF, Argentina

[5] UNCPBA, Vet Coll, Buenos Aires, DF, Argentina

[6] INTA Rafaela, EEA, Rafaela, Santa Fe, Argentina

[7] Ohio State Univ, Food Anim Hlth Res Program, Wooster, OH USA

来源：

VETERINARY MICROBIOLOGY | 2015年 / 181卷 / 3-4期

关键词：

Coronavirus; Neonatal calf diarrhea; Cross-protection; NEONATAL CALF DIARRHEA; SPIKE GLYCOPROTEIN; WINTER DYSENTERY; DAIRY-CATTLE; RESPIRATORY CORONAVIRUSES; MONOCLONAL-ANTIBODIES; CROSS-PROTECTION; S1; SUBUNIT; EGG-YOLK; RT-PCR;

D O I：

10.1016/j.vetmic.2015.10.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994-2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：221 / 229

页数：9

共 57 条

[1]

Ammar Selles Sidi Mohammed, 2014, Asian Pacific Journal of Tropical Biomedicine, V4, pS318, DOI 10.12980/APJTB.4.2014C778

[2] Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus [J].

Asano, Karen Miyuki ;

de Souza, Sibele Pinheiro ;

de Barros, Iracema Nunes ;

Ayres, Giselle Razera ;

Souza Silva, Sheila Oliveira ;

Richtzenhain, Leonardo Jose ;

Brandao, Paulo E. .

JOURNAL OF VIROLOGICAL METHODS, 2010, 169 (02) :375-379

[3] Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds [J].

Badaracco, A. ;

Garaicoechea, L. ;

Matthijnssens, J. ;

Louge Uriarte, E. ;

Odeon, A. ;

Bilbao, G. ;

Fernandez, F. ;

Parra, G. I. ;

Parreno, V. .

INFECTION GENETICS AND EVOLUTION, 2013, 18 :18-30

[4] Bovine rotavirus strains circulating in beef and dairy herds in Argentina from 2004 to 2010 [J].

Badaracco, A. ;

Garaicoechea, L. ;

Rodriguez, D. ;

Louge Uriarte, E. ;

Odeon, A. ;

Bilbao, G. ;

Galarza, R. ;

Abdala, A. ;

Fernandez, F. ;

Parreno, V. .

VETERINARY MICROBIOLOGY, 2012, 158 (3-4) :394-399

[5] A Multigene Approach for Comparing Genealogy of Betacoronavirus from Cattle and Horses [J].

Barros, Iracema N. ;

Silva, Sheila O. S. ;

Nogueira Neto, Francisco S. ;

Asano, Karen M. ;

Souza, Sibele P. ;

Richtzenhain, Leonardo J. ;

Brandao, Paulo E. .

SCIENTIFIC WORLD JOURNAL, 2013,

[6]

Bendali F, 1999, VET RES, V30, P509

[7] Evolutionary dynamics of bovine coronaviruses: natural selection pattern of the spike gene implies adaptive evolution of the strains [J].

Bidokhti, Mehdi R. M. ;

Traven, Madeleine ;

Krishna, Neel K. ;

Munir, Muhammad ;

Belak, Sandor ;

Alenius, Stefan ;

Cortey, Marti .

JOURNAL OF GENERAL VIROLOGY, 2013, 94 :2036-2049

[8] Tracing the transmission of bovine coronavirus infections in cattle herds based on S gene diversity [J].

Bidokhti, Mehdi R. M. ;

Traven, Madeleine ;

Ohlson, Anna ;

Baule, Claudia ;

Hakhverdyan, Mikhayil ;

Belak, Sandor ;

Liu, Lihong ;

Alenius, Stefan .

VETERINARY JOURNAL, 2012, 193 (02) :386-390

[9] Bovine Coronavirus Associated Syndromes [J].

Boileau, Melanie J. ;

Kapil, Sanjay .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 2010, 26 (01) :123-+

[10] Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43 [J].

Brandao, P. E. ;

Gregori, F. ;

Richtzenhain, L. J. ;

Rosales, C. A. R. ;

Villarreal, L. Y. B. ;

Jerez, J. A. .

ARCHIVES OF VIROLOGY, 2006, 151 (09) :1735-1748

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