SINE retroposons can be used in vivo as nucleation centers for de novo methylation

被引：79

作者：

Arnaud, P ^{[1
]}

Goubely, C ^{[1
]}

Pélissier, T ^{[1
]}

Deragon, JM ^{[1
]}

机构：

[1] Univ Clermont Ferrand 2, CNRS, UMR6547, F-63177 Clermont Ferrand, France

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2000年 / 20卷 / 10期

关键词：

D O I：

10.1128/MCB.20.10.3434-3441.2000

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SINEs (short interspersed elements) are an abundant class of transposable elements found in a,vide variety of eukaryotes. Using the genomic sequencing technique, we observed that plant S1 SINE retroposons mainly integrate in hypomethylated DNA regions and are targeted by methylases. Methylation can then spread from the SME into flanking genomic sequences, creating distal epigenetic modifications. This methylation spreading is vectorially directed upstream or downstream of the S1 element, suggesting that it could be facilitated when a potentially good methylatable sequence is single stranded during DNA replication, particularly when located on the lagging strand. Replication of a short methylated DNA region could thus lead to the de novo methylation of upstream or downstream adjacent sequences.

引用

页码：3434 / 3441

页数：8

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