3D Cell Culture: Recent Development in Materials with Tunable Stiffness

被引:53
作者
Baruffaldi, Desiree [1 ,2 ]
Palmara, Gianluca [1 ,2 ]
Pirri, Candido [1 ,2 ,3 ]
Frascella, Francesca [1 ,2 ]
机构
[1] Politecn Torino, Dipartimento Sci Applicata & Tecnol, I-10129 Turin, Italy
[2] Politecn Torino, PolitoBIOMed Lab, I-10129 Turin, Italy
[3] Ist Italiano Tecnol, Ctr Sustainable Futures Polito, I-10144 Turin, Italy
关键词
3D cell culture; stiffness; mechanical properties; tissue mimic; tunable materials; hydrogels; MAGNETIC-RESONANCE ELASTOGRAPHY; SHEAR-WAVE ELASTOGRAPHY; ULTRASOUND ELASTOGRAPHY; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; TRANSIENT ELASTOGRAPHY; YOUNGS MODULUS; DRUG-RESISTANCE; SILK HYDROGELS; LUNG-CANCER;
D O I
10.1021/acsabm.0c01472
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
It is widely accepted that three-dimensional cell culture systems simulate physiological conditions better than traditional 2D systems. Although extracellular matrix components strongly modulate cell behavior, several studies underlined the importance of mechanosensing in the control of different cell functions such as growth, proliferation, differentiation, and migration. Human tissues are characterized by different degrees of stiffness, and various pathologies (e.g., tumor or fibrosis) cause changes in the mechanical properties through the alteration of the extracellular matrix structure. Additionally, these modifications have an impact on disease progression and on therapy response. Hence, the development of platforms whose stiffness could be modulated may improve our knowledge of cell behavior under different mechanical stress stimuli. In this review, we have analyzed the mechanical diversity of healthy and diseased tissues, and we have summarized recently developed materials with a wide range of stiffness.
引用
收藏
页码:2233 / 2250
页数:18
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